原生荧光光谱分析揭示了不同危险程度的前列腺癌细胞系之间的光谱差异。
Native fluorescence spectroscopy reveals spectral differences among prostate cancer cell lines with different risk levels.
机构信息
City College of the City University of New York, Institute for Ultrafast Spectroscopy and Lasers, Department of Physics, New York, New York 10031, USA.
出版信息
J Biomed Opt. 2013 Aug;18(8):87002. doi: 10.1117/1.JBO.18.8.087002.
Abstract
The spectral changes of native fluorophores among normal fibroblasts and cancer cell lines of different metastatic ability are investigated by fluorescence spectroscopy. The normal (fibroblast), moderately metastatic (DU-145), and advanced metastatic (PC-3) cell lines were each selectively excited at 300 nm, and their fluorescence emission spectra are analyzed using principal component analysis to explore the differences of the relative contents of tryptophan and reduced nicotinamide adenine dinucleotide in these cell lines. The results show that the tryptophan emission featured predominantly in the fluorescence spectra of the advanced metastatic cancer cells in comparison with the moderately metastatic cancer and normal cells.
摘要
通过荧光光谱法研究了不同转移能力的正常成纤维细胞和癌细胞系中天然荧光团的光谱变化。分别在 300nm 处选择性激发正常（成纤维细胞）、中度转移（DU-145）和晚期转移（PC-3）细胞系，并使用主成分分析分析它们的荧光发射光谱，以探索这些细胞系中色氨酸和还原型烟酰胺腺嘌呤二核苷酸相对含量的差异。结果表明，与中度转移性癌细胞和正常细胞相比，晚期转移性癌细胞的荧光光谱中色氨酸发射特征更为明显。
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