Institute for Ultrafast Spectroscopy and Lasers, The Department of Electric Engineering, City University of New York, Convent Avenue at 138th Street, New York, NY 10031, USA.
Technol Cancer Res Treat. 2011 Apr;10(2):113-20. doi: 10.7785/tcrt.2012.500186.
The native fluorescence spectra of retinoic acid (RA)-treated and untreated human breast cancerous cells excited with the selective wavelengths of 300 nm and 340 nm were measured and analyzed using a blind source separation method namely Nonnegative Matrix Factorization (NMF). The results show that the fluorophores of human malignant breast cells change their compositions when they are treated with RA. The reduced contribution from tryptophan, NADH and flavin to the fluorescence of the treated breast cancerous cells was observed in comparison with that of the untreated cells. The results indicate that the decrease of adenosine triphosphate (ATP) in the RA-treated cells. The possible clinical applications of this native fluorescence study are discussed.
采用盲源分离方法（即非负矩阵分解法）对 300nm 和 340nm 选择性波长激发的维甲酸（RA）处理和未处理的人乳腺癌细胞的固有荧光光谱进行了测量和分析。结果表明，当用 RA 处理时，人恶性乳腺细胞的荧光团组成发生了变化。与未处理的细胞相比，处理后的乳腺癌细胞中色氨酸、NADH 和黄素的荧光贡献减少。结果表明 RA 处理细胞中三磷酸腺苷（ATP）的减少。讨论了这项固有荧光研究的可能临床应用。
