Pradhan A, Pal P, Durocher G, Villeneuve L, Balassy A, Babai F, Gaboury L, Blanchard L
Département de Chimie, Université de Montréal, Que, Canada.
J Photochem Photobiol B. 1995 Dec;31(3):101-12. doi: 10.1016/1011-1344(95)07178-4.
Steady state and time-resolved fluorescence spectroscopy were employed to study the fluorescence from non-metastatic, metastatic and non-tumorigenic cell lines from different species. Excitations at 310 nm and 350 nm were used to monitor tryptophan and reduced nicotinamide adenine dinucleotide (NADH) fluorescence respectively. Subtle and consistent differences were observed between different categories of cell lines. It was found that the tryptophan to NADH fluorescence intensity ratio is higher in metastatic cell lines than in non-metastatic and normal cell lines. The fluorescence decay of the tryptophan residue in different cell lines was best described by triple exponential kinetics, whereas the NADH fluorescence decay was best described by mainly double and, in some cases, triple exponential kinetics. The average fluorescence lifetimes for tryptophan were in the range 2.5-3.7 ns. The average lifetime of NADH was lower (by a factor of approximately three) in metastatic cells than in non-metastatic cells and this finding is consistent for cell lines from different origins (rat or human). Correcting the fluorescence intensity for the average fluorescence lifetime of each species and for the volume of each cell line, it was shown that the concentrations of tryptophan and NADH are consistently higher in malignant metastatic cancer cells than in non-metastatic cells.
采用稳态和时间分辨荧光光谱法研究了来自不同物种的非转移性、转移性和非致瘤性细胞系的荧光。分别用310nm和350nm的激发光监测色氨酸和还原型烟酰胺腺嘌呤二核苷酸（NADH）的荧光。在不同类别的细胞系之间观察到了细微且一致的差异。发现转移性细胞系中色氨酸与NADH荧光强度比高于非转移性和正常细胞系。不同细胞系中色氨酸残基的荧光衰减最好用三重指数动力学来描述，而NADH荧光衰减最好用主要为双重指数动力学，在某些情况下用三重指数动力学来描述。色氨酸的平均荧光寿命在2.5 - 3.7ns范围内。转移性细胞中NADH的平均寿命低于非转移性细胞（约为其三分之一），这一发现对于来自不同来源（大鼠或人类）的细胞系是一致的。对每种细胞系的平均荧光寿命和体积进行荧光强度校正后，结果表明恶性转移性癌细胞中色氨酸和NADH的浓度始终高于非转移性细胞。
