通过紫外/可见光谱法测定药物与抗体比率（DAR）。
Drug-to-antibody ratio (DAR) by UV/Vis spectroscopy.
作者信息
Chen Yan
机构信息
Protein Analytical Chemistry, Genentech Inc., South San Francisco, CA, USA.
出版信息
Methods Mol Biol. 2013;1045:267-73. doi: 10.1007/978-1-62703-541-5_16.
PMID:23913153
Abstract
UV/Vis spectroscopy is a simple and convenient method to determine protein concentrations as well as the average number of drugs that are conjugated to the antibody in an antibody-drug conjugate (ADC). Using the measured absorbances of the ADC and the extinction coefficients of the antibody and the drug, the average drug-to-antibody ratio (DAR) can be determined.
摘要
紫外/可见光谱法是一种简单便捷的方法，可用于测定蛋白质浓度以及抗体药物偶联物（ADC）中与抗体偶联的药物平均数量。利用测得的ADC吸光度以及抗体和药物的消光系数，可确定平均药物与抗体比率（DAR）。
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