通过液相色谱-电喷雾电离质谱法测定药物与抗体比率（DAR）及药物负载分布
Drug-to-antibody ratio (DAR) and drug load distribution by LC-ESI-MS.
作者信息
Basa Louisette
机构信息
Protein Analytical Chemistry, Genentech Inc., South San Francisco, CA, USA.
出版信息
Methods Mol Biol. 2013;1045:285-93. doi: 10.1007/978-1-62703-541-5_18.
This chapter describes an LC-ESI-MS method for the DAR and drug load distribution analysis that is suitable for lysine-linked ADCs. The ADC sample is desalted using a reversed-phase LC column with an acetonitrile gradient prior to online MS analysis. The MS spectrum is processed (deconvoluted) and converted to a series of zero charge state masses that corresponds to the increasing number of drugs in the ADC. Integration of the mass peak area allows the calculation of the DAR and drug load distribution of ADCs.
本章介绍了一种适用于赖氨酸连接的抗体药物偶联物（ADCs）的DAR（药物与抗体比）和药物负载分布分析的液相色谱-电喷雾电离质谱（LC-ESI-MS）方法。在进行在线质谱分析之前，使用带有乙腈梯度的反相液相色谱柱对ADC样品进行脱盐处理。对质谱图进行处理（去卷积），并转换为一系列零电荷态质量，这些质量对应于ADC中药物数量的增加。对质量峰面积进行积分可计算出ADC的DAR和药物负载分布。
Zotero 插件