Advanced Light Source Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
Nat Methods. 2013 Sep;10(9):861-4. doi: 10.1038/nmeth.2596. Epub 2013 Aug 4.
We report Fourier transform infrared spectro-microtomography, a nondestructive three-dimensional imaging approach that reveals the distribution of distinctive chemical compositions throughout an intact biological or materials sample. The method combines mid-infrared absorption contrast with computed tomographic data acquisition and reconstruction to enhance chemical and morphological localization by determining a complete infrared spectrum for every voxel (millions of spectra determined per sample).
我们报告傅里叶变换红外光谱层析成像技术，这是一种无损的三维成像方法，可以揭示完整生物或材料样本中独特化学成分的分布。该方法结合了中红外吸收对比和计算机断层扫描数据采集和重建，通过为每个体素（每个样本确定数百万个光谱）确定完整的红外光谱，来增强化学和形态学定位。
