Holman H-Y N, Martin M C, McKinney W R
Lawrence Berkeley National Laboratory, Berkeley, CA 94720 USA.
J Biol Phys. 2003 Jun;29(2-3):275-86. doi: 10.1023/A:1024465414395.
Synchrotron radiation-based Fouriertransform infrared (SR-FTIR)spectromicroscopy is a newly emergingbioanalytical and imaging tool. This uniquetechnique provides mid-infrared (IR)spectra, hence chemical information, withhigh signal-to-noise at spatial resolutionsas fine as 3 to 10 microns. Thus it enablesresearchers to locate, identify, and trackspecific chemical events within anindividual living mammalian cell. Mid-IRphotons are too low in energy (0.05-0.5eV) to either break bonds or to causeionization. In this review, we show thatthe synchrotron IR beam has no detectableeffects on the short- and long-termviability, reproductive integrity,cell-cycle progression, and mitochondrialmetabolism in living human cells, andproduces only minimal sample heating (<0.5°C). These studies haveestablished an important foundation forSR-FTIR spectromicroscopy in biological andbiomedical research.
基于同步辐射的傅里叶变换红外（SR-FTIR）光谱显微镜是一种新兴的生物分析和成像工具。这种独特的技术可提供中红外（IR）光谱，从而获得化学信息，在空间分辨率低至3至10微米时具有高信噪比。因此，它使研究人员能够在单个活的哺乳动物细胞内定位、识别和追踪特定的化学事件。中红外光子的能量太低（0.05-0.5电子伏特），既不能破坏化学键也不能引起电离。在本综述中，我们表明同步辐射红外光束对活的人类细胞的短期和长期活力、生殖完整性、细胞周期进程和线粒体代谢没有可检测到的影响，并且仅产生最小的样品加热（<0.5°C）。这些研究为SR-FTIR光谱显微镜在生物学和生物医学研究中奠定了重要基础。
