Flett Fiona, Interthal Heidrun
Institute of Cell Biology, University of Edinburgh, Edinburgh, UK.
Methods Mol Biol. 2013;1054:173-85. doi: 10.1007/978-1-62703-565-1_11.
Denaturing urea polyacrylamide gel electrophoresis (PAGE) allows the separation of linear single-stranded DNA molecules based on molecular weight. This method can be used to analyze or purify short synthesized DNA oligonucleotides or products from enzymatic reactions.In this chapter we describe how to prepare and how to run these high concentration polyacrylamide gels. We detail how to transfer a gel onto Whatman paper and how to dry it. Radiolabelled oligonucleotides are visualized by PhosphorImager technology.
变性尿素聚丙烯酰胺凝胶电泳（PAGE）可根据分子量分离线性单链DNA分子。该方法可用于分析或纯化短的合成DNA寡核苷酸或酶促反应产物。在本章中，我们描述了如何制备和运行这些高浓度聚丙烯酰胺凝胶。我们详细介绍了如何将凝胶转移到沃特曼纸上以及如何干燥它。放射性标记的寡核苷酸通过磷光成像技术进行可视化。
