Ling Y H, Ding J P, Zhang X D, Wang L J, Zhang Y H, Li Y S, Zhang Z J, Zhang X R
College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Genet Mol Res. 2013 Jun 13;12(2):1951-61. doi: 10.4238/2013.June.13.4.
MicroRNAs (miRNAs) are an important class of small noncoding RNAs that are highly conserved in plants and animals. Many miRNAs are known to mediate a myriad of cell processes, including proliferation and differentiation, via the regulation of some transcription and signaling factors, which are closely related to muscle development and disease. In this study, small RNA cDNA libraries of Boer goats were constructed. In addition, we obtained the goat muscle miRNAs by using Solexa deep-sequencing technology and analyzed these miRNA characteristics by combining it with the bioinformatics technology. Based on Solexa sequencing and bioinformatics analysis, 562 species-conserved and 5 goat genome-specific miRNAs were identified, 322 of which exceeded 100 in the expression levels. The results of real-time quantitative polymerase chain reaction from 8 randomly selected miRNAs showed that the 8 miRNAs were expressed in goat muscle, and the expression patterns were consistent with the Solexa sequencing results. The identification and characterization of miRNAs in goat muscle provide important information on the role of miRNA regulation in muscle growth and development. These data will help to facilitate studies on the regulatory roles played by miRNAs during goat growth and development.
微小RNA（miRNA）是一类重要的小非编码RNA，在动植物中高度保守。已知许多miRNA通过调控一些与肌肉发育和疾病密切相关的转录和信号因子，介导包括增殖和分化在内的众多细胞过程。在本研究中，构建了波尔山羊的小RNA cDNA文库。此外，我们利用Solexa深度测序技术获得了山羊肌肉miRNA，并结合生物信息学技术分析了这些miRNA的特征。基于Solexa测序和生物信息学分析，鉴定出562种物种保守的miRNA和5种山羊基因组特异性miRNA，其中322种的表达水平超过100。对随机选择的8种miRNA进行实时定量聚合酶链反应的结果表明，这8种miRNA在山羊肌肉中表达，且表达模式与Solexa测序结果一致。山羊肌肉中miRNA的鉴定和特征分析为miRNA调控在肌肉生长发育中的作用提供了重要信息。这些数据将有助于促进对miRNA在山羊生长发育过程中所起调控作用的研究。
