miR-619-5p在人类及直系同源基因mRNA中的结合位点
The Binding Sites of miR-619-5p in the mRNAs of Human and Orthologous Genes.
作者信息
Atambayeva Shara, Niyazova Raigul, Ivashchenko Anatoliy, Pyrkova Anna, Pinsky Ilya, Akimniyazova Aigul, Labeit Siegfried
机构信息
SRI Of Biology and Biotechnology Problems, Al-Farabi Kazakh National University, Almaty, Kazakhstan.
Institute for Anaesthesiology and Intensive Operative Care Medical Faculty Mannheim, Mannheim, Germany.
出版信息
BMC Genomics. 2017 Jun 1;18(1):428. doi: 10.1186/s12864-017-3811-6.
BACKGROUND
Normally, one miRNA interacts with the mRNA of one gene. However, there are miRNAs that can bind to many mRNAs, and one mRNA can be the target of many miRNAs. This significantly complicates the study of the properties of miRNAs and their diagnostic and medical applications.
RESULTS
The search of 2,750 human microRNAs (miRNAs) binding sites in 12,175 mRNAs of human genes using the MirTarget program has been completed. For the binding sites of the miR-619-5p the hybridization free energy of the bonds was equal to 100% of the maximum potential free energy. The mRNAs of 201 human genes have complete complementary binding sites of miR-619-5p in the 3'UTR (214 sites), CDS (3 sites), and 5'UTR (4 sites). The mRNAs of CATAD1, ICA1L, GK5, POLH, and PRR11 genes have six miR-619-5p binding sites, and the mRNAs of OPA3 and CYP20A1 genes have eight and ten binding sites, respectively. All of these miR-619-5p binding sites are located in the 3'UTRs. The miR-619-5p binding site in the 5'UTR of mRNA of human USP29 gene is found in the mRNAs of orthologous genes of primates. Binding sites of miR-619-5p in the coding regions of mRNAs of C8H8orf44, C8orf44, and ISY1 genes encode the WLMPVIP oligopeptide, which is present in the orthologous proteins. Binding sites of miR-619-5p in the mRNAs of transcription factor genes ZNF429 and ZNF429 encode the AHACNP oligopeptide in another reading frame. Binding sites of miR-619-5p in the 3'UTRs of all human target genes are also present in the 3'UTRs of orthologous genes of mammals. The completely complementary binding sites for miR-619-5p are conservative in the orthologous mammalian genes.
CONCLUSIONS
The majority of miR-619-5p binding sites are located in the 3'UTRs but some genes have miRNA binding sites in the 5'UTRs of mRNAs. Several genes have binding sites for miRNAs in the CDSs that are read in different open reading frames. Identical nucleotide sequences of binding sites encode different amino acids in different proteins. The binding sites of miR-619-5p in 3'UTRs, 5'UTRs and CDSs are conservative in the orthologous mammalian genes.
背景
通常，一个微小RNA（miRNA）与一个基因的信使核糖核酸（mRNA）相互作用。然而，存在一些能与多个mRNA结合的miRNA，并且一个mRNA可以是多个miRNA的靶标。这显著地使对miRNA特性及其诊断和医学应用的研究变得复杂。
结果
使用MirTarget程序在人类基因的12175个mRNA中搜索2750个人类微小RNA（miRNA）的结合位点的工作已经完成。对于miR - 619 - 5p的结合位点，键的杂交自由能等于最大潜在自由能的100%。201个人类基因的mRNA在3'非翻译区（UTR）（214个位点）、编码区（CDS）（3个位点）和5'UTR（4个位点）中具有miR - 619 - 5p的完全互补结合位点。CATAD1、ICA1L、GK5、POLH和PRR11基因的mRNA具有6个miR - 619 - 5p结合位点，OPA3和CYP20A1基因的mRNA分别具有8个和10个结合位点。所有这些miR - 619 - 5p结合位点都位于3'UTRs中。人类USP29基因mRNA的5'UTR中的miR - 619 - 5p结合位点在灵长类同源基因的mRNA中被发现。C8H8orf44、C8orf44和ISY1基因mRNA编码区中的miR - 619 - 5p结合位点编码WLMPVIP寡肽，该寡肽存在于同源蛋白中。转录因子基因ZNF429和ZNF429的mRNA中的miR - 619 - 5p结合位点在另一个阅读框中编码AHACNP寡肽。所有人类靶基因3'UTRs中的miR - 619 - 5p结合位点也存在于哺乳动物同源基因的3'UTRs中。miR - 619 - 5p的完全互补结合位点在哺乳动物同源基因中是保守的。
结论
大多数miR - 619 - 5p结合位点位于3'UTRs中，但一些基因在mRNA的5'UTRs中有miRNA结合位点。几个基因在CDSs中有miRNA结合位点，这些位点在不同的开放阅读框中被读取。结合位点相同的核苷酸序列在不同蛋白质中编码不同的氨基酸。miR - 619 - 5p在3'UTRs, 5'UTRs和CDSs中的结合位点在哺乳动物同源基因中是保守的。
Zotero 插件