Helmers Renée, Milstein Dan M J, van Hulst Robert A, de Lange Jan
Department of Oral and Maxillofacial Surgery, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
Head Neck. 2014 Sep;36(9):1241-7. doi: 10.1002/hed.23437. Epub 2013 Nov 6.
Hyperbaric oxygen therapy (HBOT) is thought to promote vascular regeneration in wounds. The purpose of this study was to investigate the role of HBOT in advancing vascular regeneration in healing oral mucosal surgical flaps.
A palatine partial-thickness mucosal flap was raised in 10 male-specific pathogen-free New Zealand White rabbits. Randomized into 2 groups of 5 animals each (control and HBOT), functional capillary density was measured preoperatively (baseline), and immediately postoperatively until day 21 using sidestream dark-field video microscopy. Ten HBOT sessions were administered over the course of 2 weeks at 2.5 atmospheres (2.5 bar O2 /90 minutes).
Repeated measures analysis of variance was used to compare the HBOT and control group on the sequential functional capillary density measurements. A significant interaction effect was present between time and group (F [8, 64] = 9.60; p < .0001) resulting from a significant increase in microcirculation in the HBOT group relative to the control group on days 7, 9, and 11.
Our results suggest that HBOT is capable of advancing wound vascular regeneration in healing keratinized oral mucosal flaps.
高压氧疗法（HBOT）被认为可促进伤口血管再生。本研究的目的是探讨HBOT在促进愈合中的口腔黏膜手术皮瓣血管再生方面的作用。
在10只雄性无特定病原体的新西兰白兔身上掀起腭部部分厚度黏膜瓣。随机分为两组，每组5只动物（对照组和HBOT组），术前（基线）、术后即刻直至第21天使用侧流暗视野视频显微镜测量功能性毛细血管密度。在2周内以2.5个大气压（2.5巴氧气/90分钟）进行10次HBOT治疗。
采用重复测量方差分析比较HBOT组和对照组在连续功能性毛细血管密度测量上的差异。时间和组之间存在显著的交互作用（F [8, 64] = 9.60；p <.0001），这是由于HBOT组在第7、9和11天相对于对照组微循环显著增加所致。
我们的结果表明，HBOT能够促进愈合中的角化口腔黏膜皮瓣伤口的血管再生。
