使用三种不同指标评估艾美耳球虫属田间分离株对抗球虫药的耐药性。
Evaluating the resistance of eimeria spp. Field isolates to anticoccidial drugs using three different indices.
作者信息
Arabkhazaeli F, Modrisanei M, Nabian S, Mansoori B, Madani A
机构信息
Department of Pathobiology, Faculty of Veterinary Medicine, University of Tehran, Iran.
出版信息
Iran J Parasitol. 2013 Apr;8(2):234-41.
BACKGROUND
In this study, the presence of resistance to diclazuril, amprolium+ethopabate and salinomycin, representing some of the commonest anticoccidials in Iran's poultry industry, against three mixed Eimeria field isolates were investigated.
METHODS
Three Eimeria field isolates, collected from typical broiler farms in Iran, were propagated once, inoculated to 480 broilers, comprising 30 chicks in each treatment. The non-medicated or medicated diets containing one of the above mentioned anticoccidials were provided ad-lib. Drug efficacy was determined using the Global index (GI), Anticoccidial Sensitivity Test (AST) and Optimum Anticoccidial Activity (OAA).
RESULTS
None of the field isolates were fully sensitive to the selected anticoccidials. All isolates showed reduced sensitivity/partial resistance to salinomycin. Resistance to amprolium+ethopabate was evident and partial to complete resistance was recorded for diclazuril.
CONCLUSION
Limited efficacy of the selected anticoccidials is obvious. Considering the cost of continuous use of anticoccidials in the field, altering the prevention strategy and rotation of the anticoccidials with better efficacy, would prevent further economic losses induced by coccidiosis.
背景
在本研究中，调查了伊朗家禽业中一些最常用的抗球虫药，即地克珠利、氨丙啉+乙氧酰胺苯甲酯和盐霉素，对三种艾美耳球虫混合野外分离株的耐药性情况。
方法
从伊朗典型的肉鸡养殖场收集了三种艾美耳球虫野外分离株，传代一次后接种到480只肉鸡中，每种处理包含30只雏鸡。自由采食含有上述抗球虫药之一的未用药或用药日粮。使用全球指数（GI）、抗球虫药敏感性试验（AST）和最佳抗球虫活性（OAA）来确定药物疗效。
结果
所有野外分离株对所选抗球虫药均未完全敏感。所有分离株对盐霉素的敏感性均降低/部分耐药。对氨丙啉+乙氧酰胺苯甲酯的耐药性明显，地克珠利存在部分至完全耐药。
结论
所选抗球虫药的疗效有限是显而易见的。考虑到在野外持续使用抗球虫药的成本，改变预防策略并轮换使用疗效更好的抗球虫药，将防止球虫病造成进一步的经济损失。
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