Al-Hindi Adnan I, Shammala Basma M Abu
Department of Biology, Faculty of Science, the Islamic University of Gaza, P.O.Box 108, Gaza, Palestine.
Iran J Parasitol. 2013 Apr;8(2):249-55.
The aim of this study was to detect Dientamoeba fragilis by iron haematoxylin stain, as well as its prevalence, and association between D. fragilis infection and diarrhoea among patients attending Al-Nuseirate Refugee Camp Clinic, Gaza Strip.
A cross-sectional study was conducted among 319 children and adults with age ranges from (1 to 75) years old, attending Al-Nussirat Clinic, and who were complaining from clinical symptoms, like diarrhoea and abdominal pain.
28 individuals were infected with D. fragilis with a prevalence of 8.8%. The detection of 28 cases infected with D. fragilis was proved using iron haematoxylin stain, but no case was detected by direct smear or formal-ether sedimentation technique. The most frequent symptoms were abdominal pain (96.4%) and diarrhoea (71.4%) in patients with diantamoebiasis and this was statistically significant (P= 0.03). Co-infection between D. fragilis and Entamoeba histolytica/dispar was 50% and between D. fragilis and Giardia lamblia was 7.1%.
D. fragilis was present in the patients stool samples and was detected and proved using iron haematoxylin stain.
本研究旨在通过铁苏木精染色检测脆弱双核阿米巴，及其在加沙地带努赛拉特难民营诊所就诊患者中的患病率，以及脆弱双核阿米巴感染与腹泻之间的关联。
对319名年龄在1至75岁之间、在努赛拉特诊所就诊且有腹泻和腹痛等临床症状的儿童和成人进行了一项横断面研究。
28人感染脆弱双核阿米巴，患病率为8.8%。使用铁苏木精染色证实了28例脆弱双核阿米巴感染病例，但直接涂片或甲醛-乙醚沉淀技术未检测到病例。双核阿米巴病患者最常见的症状是腹痛（96.4%）和腹泻（71.4%），这具有统计学意义（P = 0.03）。脆弱双核阿米巴与溶组织内阿米巴/迪斯帕内阿米巴的合并感染率为50%，与蓝氏贾第鞭毛虫的合并感染率为7.1%。
脆弱双核阿米巴存在于患者粪便样本中，并通过铁苏木精染色检测到并得到证实。
