Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki, Japan.
J Int Soc Sports Nutr. 2013 Aug 6;10(1):35. doi: 10.1186/1550-2783-10-35.
Some studies have shown that dietary hydrolyzed collagen peptides (HC) effectively prevent age-related bone loss. However, it is not known whether the intake of HC also has positive effect on bone mass or strength when combined with exercise during growth phase.
We examined the effects of 11 weeks of HC intake and running exercise on bone mass and strength in growing rats. Rats were randomized into four groups, the 20% casein group (Casein20), the 40% casein group (Casein40), the 20% HC group (HC20), and the 40% HC group (HC40). Each group was further divided into exercise groups (Casein20 + Ex, Casein40 + Ex, HC20 + Ex, HC40 + Ex) and non-exercise group (Casein20, Casein40, HC20, HC40). In the HC intake groups, 30% of casein protein was replaced with HC. Exercise group rats were trained 6 days per week on a treadmill (25-30 m/min, 60 min) for 60 days. After being sacrificed, their bone mineral content (BMC) and bone strength were evaluated.
Exercise and dietary HC effects were observed in the adjusted BMC of lumbar spine and tibia among the 20% protein groups (p < 0.001 for exercise; p < 0.05 for dietary HC, respectively). These effects were also noted in the adjusted wet weight and dry weight of femur among the 20% protein groups (p < 0.001, p < 0.01 for exercise; p < 0.01, p < 0.001 for dietary HC, respectively). On the other hand, in adjusted bone breaking force and energy, dietary HC effect was not significant. Among the 40% protein groups, similar results were obtained in the adjusted BMC, femoral weight, bone breaking force, and energy. There were no differences between the 20% protein groups and the 40% protein groups.
The present study demonstrated that moderate HC intake (where the diet contains 20% protein, of which 30% is HC) increased bone mass during growth period and further promoted the effect of running exercise. On the other hand, a higher HC intake (where the diet contains 40% protein, of which 30% is HC) had no more beneficial effect on bone mass than the moderate HC intake.
一些研究表明，膳食水解胶原蛋白肽（HC）可有效预防与年龄相关的骨丢失。然而，当在生长阶段结合运动摄入 HC 时，其对骨量或骨强度是否有积极影响尚不清楚。
我们研究了 11 周 HC 摄入和跑步运动对生长大鼠骨量和骨强度的影响。将大鼠随机分为四组：20%乳清蛋白组（Casein20）、40%乳清蛋白组（Casein40）、20% HC 组（HC20）和 40% HC 组（HC40）。每组进一步分为运动组（Casein20+Ex、Casein40+Ex、HC20+Ex、HC40+Ex）和非运动组（Casein20、Casein40、HC20、HC40）。在 HC 摄入组中，用 30%乳清蛋白替代 30%的酪蛋白。运动组大鼠每周在跑步机上训练 6 天（25-30m/min，60min），共 60 天。处死大鼠后，评估其骨矿物质含量（BMC）和骨强度。
在 20%蛋白质组中，运动和膳食 HC 对腰椎和胫骨的 BMC 调整有影响（运动组 p<0.001；膳食 HC 组 p<0.05）。在 20%蛋白质组中，股骨湿重和干重的调整也有影响（运动组 p<0.001，p<0.01；膳食 HC 组 p<0.01，p<0.001）。另一方面，膳食 HC 对骨断裂力和能量的影响不显著。在 40%蛋白质组中，调整后的 BMC、股骨重量、骨断裂力和能量也得到了类似的结果。20%蛋白质组和 40%蛋白质组之间没有差异。
本研究表明，适度 HC 摄入（饮食中含有 20%蛋白质，其中 30%为 HC）可增加生长期间的骨量，并进一步促进跑步运动的效果。另一方面，较高的 HC 摄入（饮食中含有 40%蛋白质，其中 30%为 HC）对骨量的益处并不大于适度 HC 摄入。
