Douglass K D, Cobb C M, Berkstein S, Killoy W J
Department of Periodontics, School of Dentistry, Univerity of Missouri-Kansas City.
J Periodontol. 1990 Aug;61(8):475-84. doi: 10.1902/jop.1990.61.8.475.
The purpose of this investigation was to characterize by scanning electron microscopy, the microbial morphotypes associated with the roots of teeth from patients exhibiting localized juvenile periodontitis (LJP). Eighteen teeth were examined from 10 adolescent patients who satisfied the standard clinical diagnostic criteria for LJP. The microscopic evaluation was facilitated by dividing that portion of the root exposed to the periodontal pocket into coronal, middle, and apical one-thirds. Controls consisted of an equal number of teeth matched as to pocket depth and anatomical type obtained from patients exhibiting adult periodontitis (AP). All specimens were coded and examiners were unaware of their origins. Results of the SEM evaluation revealed little difference in microbial morphotypes comprising the root associated plaque in the coronal one-third of LJP specimens when compared to the AP controls. The dominant microbial morphotypes were cocci, short and long rods, filamentous microorganisms, and spirochetes. However, microbial plaque located in the middle and apical one-third root zones of LJP specimens was distinctly different than that of AP specimens. In the deeper pocket zones the dominant microbial morphotypes in LJP specimens were limited to cocci, short rods, coccobacilli, and various sized spirochetes. In contrast, AP specimens exhibited a collection of microbial morphotypes consisting of cocci, short rods, long rods, filamentous organisms and spirochetes. Calculus was found on the root surfaces of all specimens regardless of disease category although, as a group, the LJP specimens featured lesser amounts. Although areas of root resorption were common in both LJP and AP groups, they were more frequently observed in LJP specimens, likely associated with the generally less confluent deposits of calculus and plaque.
本研究的目的是通过扫描电子显微镜对与局限性青少年牙周炎（LJP）患者牙齿根部相关的微生物形态类型进行表征。从10名符合LJP标准临床诊断标准的青少年患者中检查了18颗牙齿。通过将暴露于牙周袋的牙根部分分为冠部、中部和根尖三分之一来便于进行显微镜评估。对照组由从患有成人牙周炎（AP）的患者中获取的数量相等的牙齿组成，这些牙齿在牙周袋深度和解剖类型方面相匹配。所有标本均进行编码，检查人员不知道其来源。扫描电子显微镜评估结果显示，与AP对照组相比，LJP标本冠部三分之一处与牙根相关菌斑的微生物形态类型差异不大。主要的微生物形态类型为球菌、短杆菌和长杆菌、丝状微生物和螺旋体。然而，LJP标本牙根中部和根尖三分之一区域的微生物菌斑与AP标本明显不同。在较深的牙周袋区域，LJP标本中的主要微生物形态类型仅限于球菌、短杆菌、球杆菌和各种大小的螺旋体。相比之下，AP标本表现出由球菌、短杆菌、长杆菌、丝状生物和螺旋体组成的多种微生物形态类型。所有标本的牙根表面均发现有牙石，无论疾病类型如何，尽管作为一个整体，LJP标本中的牙石量较少。虽然牙根吸收区域在LJP组和AP组中都很常见，但在LJP标本中更频繁地观察到，这可能与牙石和菌斑的沉积通常不太融合有关。
