Asikainen S, Jousimies-Somer H, Kanervo A, Summanen P
J Periodontol. 1987 Apr;58(4):224-30. doi: 10.1902/jop.1987.58.4.224.
Subgingival microflora in localized juvenile periodontitis (LJP) was examined by culture of certain bacterial species and by direct dark-field microscopy in affected and unaffected periodontal pockets of 11 patients and in their age- and sex-matched controls. Only Actinobacillus actinomycetemcomitans (A.a.) correlated to periodontal destruction and occurred most frequently at the affected LJP sites. A.a., Bacteroides intermedius and Fusobacterium nucleatum were isolated in proportions greater than 5% of the flora at the affected LJP sites, but various Actinomyces spp. at the unaffected and control sites. The proportions of straight rods, fusiforms and small spirochetes correlated to gingival bleeding tendency and were higher at the affected LJP sites than at the control sites. Certain nonpigmented Bacteroides spp. and Eikenella corrodens were never isolated when only cocci were seen at dark-field microscopy. The more complex the flora, the more frequently B. intermedius occurred. The results support a significant role for A.a. in LJP and indicate that certain bacterial species appear to prefer occurring in the complex composition of subgingival microflora.
通过对特定细菌种类进行培养以及直接暗视野显微镜检查，对11例局限性青少年牙周炎（LJP）患者患牙和未患牙的牙周袋以及年龄和性别匹配的对照组进行研究，以检测龈下微生物群。只有伴放线放线杆菌（A.a.）与牙周破坏相关，且在受影响的LJP部位出现频率最高。在受影响的LJP部位，A.a.、中间普氏菌和具核梭杆菌的分离比例超过菌群的5%，但在未受影响部位和对照部位则分离出各种放线菌属。直杆菌、梭形菌和小螺旋体的比例与牙龈出血倾向相关，且在受影响的LJP部位高于对照部位。当暗视野显微镜下仅见球菌时，从未分离出某些非色素普氏菌属和腐蚀艾肯菌。菌群越复杂，中间普氏菌出现的频率越高。结果支持A.a.在LJP中起重要作用，并表明某些细菌种类似乎更倾向于出现在复杂的龈下微生物群组成中。
