College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China.
Int J Biol Macromol. 2013 Oct;61:347-52. doi: 10.1016/j.ijbiomac.2013.07.013. Epub 2013 Aug 2.
As the major constituent of Radix Astragali, Astragalus polysaccharide (APS) is known for its anti-inflammation and immunomodulatory functions. The objective of this study was to investigate the effect of APS on inflammatory response and structural changes in lipopolysaccharide (LPS)-infected Caco2 cells. Caco2 cells were co-cultured with APS and LPS, with APS added after the addition of LPS (post-addition), before the addition of LPS (pre-addition), or simultaneously with the addition of LPS (simultaneous addition). The mRNA expression of inflammatory indicators and tight junctions was measured by RT-qPCR. Short circuit current (Isc) was recorded by an Ussing chamber system. Addition of APS significantly down-regulated the expression of TNF-α, IL-1β and IL-8 (P<0.05) and the Isc levels (P<0.05) of LPS-infected Caco2 cells for all three administration treatments. The minimum anti-inflammatory concentration of APS was 50, 100, and 100 μg/mL for pre-, post-, and simultaneous additions of APS, respectively. The mRNA expression of zonula occludens-1 (ZO-1) and occludin was significantly up-regulated for post- and pre-additions of APS, respectively (P<0.05). Results suggested that APS had anti-inflammatory and structure protective properties for LPS-infected Caco2 cells, and may be used as a preventative treatment for intestine cells.
作为黄芪的主要成分，黄芪多糖（APS）以其抗炎和免疫调节功能而闻名。本研究旨在探讨 APS 对脂多糖（LPS）感染的 Caco2 细胞炎症反应和结构变化的影响。将 Caco2 细胞与 APS 和 LPS 共培养，APS 分别在 LPS 加入后（后加入）、加入前（前加入）或同时加入（同时加入）时添加。通过 RT-qPCR 测量炎症指标和紧密连接的 mRNA 表达。通过 Ussing 室系统记录短路电流（Isc）。APS 的添加显著下调了 LPS 感染的 Caco2 细胞中 TNF-α、IL-1β 和 IL-8 的表达（P<0.05）和 Isc 水平（P<0.05）。APS 的最小抗炎浓度分别为 LPS 感染的 Caco2 细胞前、后和同时添加 APS 的 50、100 和 100μg/mL。APS 的后和前添加分别显著上调了紧密连接蛋白-1（ZO-1）和封闭蛋白的 mRNA 表达（P<0.05）。结果表明，APS 对 LPS 感染的 Caco2 细胞具有抗炎和结构保护作用，可作为肠细胞的预防治疗药物。
