College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China; College of Science, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China.
Int J Biol Macromol. 2015 May;76:188-94. doi: 10.1016/j.ijbiomac.2015.02.040. Epub 2015 Mar 4.
This study evaluates the immunomodulating activities of Astragalus polysaccharide (APS) and sulfated APS (SAPS) in LPS-infected broiler chicks. SAPS was derived using the classic chlorosulfonic acid-pyridine method. On day 16, the birds were injected intramuscularly with 0.5 mL of either saline, APS (4 or 8 mg/kg of body weight (BW), shorten as APS-4 or APS-8) or SAPS (4 or 8 mg/kg of BW, shorten as SAPS-4 or SAPS-8) once a day for three successive days. On days 19 and 20, the birds were intraperitoneally injected with 0.5 mL of LPS (1mg/kg of BW). Saline was used as blank control. Compared with the blank control, LPS-treated birds showed lower daily body weight gain (BWG), average daily feed intake (ADFI), villus height and intraepithelial lymphocytes (IEL) number in jejunum, and higher feed conversion ratio (FCR, feed:gain), spleen index, plasma NO concentration, blood heterophil:lymphocyte (H:L) ratio, and the production of NO in the blood T lymphocytes. Compared with the LPS group, birds in APS-4, SAPS-4 and SAPS-8 groups showed decreased FCR (P<0.05). Moreover, SAPS increased BWG and jejunal villus height (P<0.05) at 8 mg/kg BW. Plasma NO concentration was lower in APS-8 group than that in LPS group (P<0.05). Both APS-8 and SAPS-8 treatments elevated the number of jejunal IEL (P<0.05), and decreased blood H:L ratio (P<0.05), respectively. Administration of APS or SAPS did not affect the ADFI, immune organ index, crypt depth and mucosal thickness of the jejunum, and the number of goblet cell. Our findings suggested that APS and SAPS possessed dose-dependent growth-promoting and immunomodulating effect, and was a potential development direction for immunomodulator under early LPS stimulation condition.
本研究评估了黄芪多糖（APS）和硫酸化 APS（SAPS）在 LPS 感染肉鸡中的免疫调节活性。SAPS 是使用经典的氯磺酸-吡啶法制备的。在第 16 天，鸡肌肉注射 0.5 mL 生理盐水、APS（4 或 8mg/kg 体重（BW），简称 APS-4 或 APS-8）或 SAPS（4 或 8mg/kg BW，简称 SAPS-4 或 SAPS-8），每天一次，连续 3 天。在第 19 天和第 20 天，鸡腹腔内注射 0.5 mL LPS（1mg/kg BW）。生理盐水作为空白对照。与空白对照组相比，LPS 处理组鸡的日增重（BWG）、平均日采食量（ADFI）、空肠绒毛高度和上皮内淋巴细胞（IEL）数量较低，饲料转化率（FCR，饲料：增重）、脾脏指数、血浆 NO 浓度、血液嗜中性粒细胞：淋巴细胞（H:L）比值和血液 T 淋巴细胞中 NO 的产生较高。与 LPS 组相比，APS-4、SAPS-4 和 SAPS-8 组的 FCR 降低（P<0.05）。此外，SAPS 以 8mg/kg BW 增加了 BWG 和空肠绒毛高度（P<0.05）。与 LPS 组相比，APS-8 组血浆 NO 浓度较低（P<0.05）。APS-8 和 SAPS-8 处理均增加了空肠 IEL 数量（P<0.05），降低了血液 H:L 比值（P<0.05）。APS 或 SAPS 的给药并不影响 ADFI、免疫器官指数、隐窝深度和空肠粘膜厚度以及杯状细胞数量。我们的研究结果表明，APS 和 SAPS 具有剂量依赖性的促生长和免疫调节作用，是 LPS 早期刺激条件下免疫调节剂的潜在发展方向。
