Lukkahatai Nada, Patel Sajni, Gucek Marjan, Hsiao Chao-Pin, Saligan Leorey N
National Institute of Nursing Research, National Institutes of Health, Bethesda, Maryland, USA.
National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA.
J Pain Symptom Manage. 2014 Apr;47(4):748-756.e4. doi: 10.1016/j.jpainsymman.2013.05.016. Epub 2013 Jul 31.
Fatigue is the most distressing side effect of radiation therapy, and its progression etiology is unknown.
This study describes proteome changes from sera of fatigued men with non-metastatic prostate cancer receiving external beam radiation therapy (EBRT).
Fatigue scores, measured by the Functional Assessment of Chronic Illness Therapy-Fatigue, and serum were collected from 12 subjects at baseline (before EBRT) and at midpoint (Day 21) of EBRT. Depleted sera from both time points were analyzed using two-dimensional difference gel electrophoresis, and up/down regulated proteins were identified using liquid chromatography-tandem mass spectrometry. Western blot analyses confirmed the protein changes observed.
Results showed that apolipoprotein (Apo)A1, ApoE, and transthyretin (TTR) consistently changed from baseline (Day 0) to midpoint (Day 21). The mean ApoE level of subjects with high change in fatigue (HF: n = 9) increased significantly from baseline to midpoint and were higher than in subjects with no change in fatigue. The mean ApoA1 level was higher in HF subjects at baseline and at midpoint than in no fatigue subjects at both time points. The mean TTR level of no fatigue subjects was higher at baseline and midpoint than in HF subjects.
These ApoE, ApoA1, and TTR results may assist in understanding pathways that can explain fatigue progression etiology in this clinical population.
疲劳是放射治疗最令人痛苦的副作用，其进展病因尚不清楚。
本研究描述了接受外照射放疗（EBRT）的非转移性前列腺癌疲劳男性患者血清中的蛋白质组变化。
采用慢性病治疗功能评估-疲劳量表测量疲劳评分，并在基线（EBRT前）和EBRT中点（第21天）从12名受试者中采集血清。使用二维差异凝胶电泳分析两个时间点的去除蛋白血清，并使用液相色谱-串联质谱法鉴定上调/下调的蛋白质。蛋白质印迹分析证实了观察到的蛋白质变化。
结果显示，载脂蛋白（Apo）A1、ApoE和转甲状腺素蛋白（TTR）从基线（第0天）到中点（第21天）持续变化。疲劳变化较大的受试者（HF：n = 9）的平均ApoE水平从基线到中点显著增加，且高于疲劳无变化的受试者。HF受试者在基线和中点时的平均ApoA1水平高于两个时间点的无疲劳受试者。无疲劳受试者的平均TTR水平在基线和中点时高于HF受试者。
这些ApoE、ApoA1和TTR的结果可能有助于理解可以解释该临床人群疲劳进展病因的途径。
