Department of Nephrology, The First Affiliated Hospital, Sun Yat-sen University, Key Laboratory of Nephrology, Ministry of Health, Guangzhou, Guangdong, China.
Biochem Biophys Res Commun. 2013 Aug 30;438(3):473-8. doi: 10.1016/j.bbrc.2013.07.116. Epub 2013 Aug 2.
Peritoneal membranes can be categorized as high, high average, low average, and low transporters, based on the removal or transport rate of solutes. In this study, we used proteomic analysis to determine the differences in proteins removed by different types of peritoneal membranes. Peritoneal transport characteristics in patients who received peritoneal dialysis therapy were assessed by a peritoneal equilibration test. Two-dimensional differential gel electrophoresis technology followed by quantitative analysis was performed to study the variation in protein expression from peritoneal dialysis effluents (PDE) among different groups. Proteins were identified by MALDI-TOF-MS/MS analyses. Further validation in PDE or serum was performed utilizing ELISA analysis. Proteomics analysis revealed ten protein spots with significant differences in intensity levels among different groups, including vitamin D-binding protein, complement C3, apolipoprotein-A1, complement factor C4A, haptoglobin, alpha-1 antitrypsin, immunoglobulin kappa light chain, alpha-2-microglobulin, retinol-binding protein 4 and transthyretin. The levels of vitamin D-binding protein, complement C3, and apolipoprotein-A1 in PDE derived from different groups were greatly varied (P<0.05). However, no significant difference was found in the serum levels of these proteins among different groups (P>0.05 for all groups). This study provides a novel overview of the differences in PDE proteomes of four types of peritoneal membranes. Vitamin D-binding protein, complement C3, and apolipoprotein-A1 showed enhanced expression in PDE of patients with high transporter.
腹膜可以根据溶质的清除或转运率分为高、高平均、低平均和低转运体。在这项研究中，我们使用蛋白质组学分析来确定不同类型腹膜清除的蛋白质差异。通过腹膜平衡试验评估接受腹膜透析治疗患者的腹膜转运特性。采用二维差异凝胶电泳技术结合定量分析研究不同组间腹膜透析流出液（PDE）中蛋白质表达的变化。通过 MALDI-TOF-MS/MS 分析鉴定蛋白质。进一步利用 ELISA 分析在 PDE 或血清中进行验证。蛋白质组学分析显示，不同组间有 10 个蛋白斑点的强度水平存在显著差异，包括维生素 D 结合蛋白、补体 C3、载脂蛋白 A1、补体因子 C4A、触珠蛋白、α-1 抗胰蛋白酶、免疫球蛋白κ轻链、α-2-微球蛋白、视黄醇结合蛋白 4 和转甲状腺素蛋白。来源于不同组的 PDE 中维生素 D 结合蛋白、补体 C3 和载脂蛋白 A1 的水平差异很大（P<0.05）。然而，不同组间血清中这些蛋白质的水平没有显著差异（所有组 P>0.05）。这项研究提供了对四种类型腹膜 PDE 蛋白质组差异的新概述。维生素 D 结合蛋白、补体 C3 和载脂蛋白 A1 在高转运体患者的 PDE 中表达增强。
