Department of Nephrology, Chi-Mei Medical Center, Tainan, Taiwan.
Nephrol Dial Transplant. 2010 Jun;25(6):1955-63. doi: 10.1093/ndt/gfp696. Epub 2010 Jan 6.
Since the mechanism of comorbidity and mortality in peritoneal dialysis is unclear, a comparison of peritoneal dialysate and normal peritoneal fluid may provide clues to the biological and pathological processes involved in peritoneal damage.
Peritoneal dialysate and control samples were collected from five diabetes mellitus (DM) patients and two patients receiving laparoscopic cholecystectomy. Proteins were separated by two-dimensional gel electrophoresis (2D-GE). After image analysis, altered gel spots between these two sample groups were subjected to tryptic digestion and mass spectrometry analysis. The results were searched against the NCBI database.
A total of 26 protein spots were considered altered in 2D-GE between the two sample groups. After western blotting confirmation, vitamin D-binding protein, haptoglobin and alpha-2-microglobulin were at higher levels in the DM samples, while complement C4-A and IGK@ protein were at lower levels compared to the control samples.
The loss of vitamin D-binding protein, haptoglobin and alpha-2-microglobulin may be due to a change in the permeability of the peritoneal membrane to middle-sized proteins or leakage from peritoneal inflammation. Lower levels of complement C4-A in dialysate may shed light on the beginning of peritoneal membrane scleroses.
由于腹膜透析中合并症和死亡率的机制尚不清楚，将腹膜透析液与正常腹膜液进行比较，可能为腹膜损伤涉及的生物学和病理学过程提供线索。
从五名糖尿病（DM）患者和两名接受腹腔镜胆囊切除术的患者中收集腹膜透析液和对照样本。通过二维凝胶电泳（2D-GE）分离蛋白质。在图像分析之后，对这两个样本组之间发生改变的凝胶斑点进行胰酶消化和质谱分析。结果与 NCBI 数据库进行了搜索。
在两个样本组之间的 2D-GE 中，共发现 26 个蛋白点发生改变。经 Western blot 验证，DM 样本中的维生素 D 结合蛋白、触珠蛋白和α2-微球蛋白水平较高，而补体 C4-A 和 IGK@蛋白水平较低。
维生素 D 结合蛋白、触珠蛋白和α2-微球蛋白的丢失可能是由于中分子量蛋白质的腹膜通透性改变或腹膜炎症渗漏所致。透析液中补体 C4-A 水平降低可能表明腹膜膜硬化的开始。
