将谷氨酸棒杆菌进行化学诱变以构建甲醇抗性突变株，使其能够利用含甲醇的甘油生产甘油酸。
Chemical mutagenesis of Gluconobacter frateurii to construct methanol-resistant mutants showing glyceric acid production from methanol-containing glycerol.
机构信息
Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5-2, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5-2, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
出版信息
J Biosci Bioeng. 2014 Feb;117(2):197-199. doi: 10.1016/j.jbiosc.2013.07.004. Epub 2013 Aug 2.
To produce glyceric acid (GA) from methanol-containing glycerol, resistance to methanol of Gluconobacter frateurii NBRC103465 was improved by chemical mutagenesis using N-methyl-N'-nitro-N-nitrosoguanidine. The obtained mutant Gf398 produced 6.3 g/L GA in 5% (v/v) methanol-containing 17% (w/v) glycerol medium, in which the wild-type strain neither grew nor produced GA.
为了从含有甲醇的甘油中生产甘油酸（GA），使用 N-甲基-N'-硝基-N-亚硝基胍对弗氏葡萄糖杆菌 NBRC103465 的甲醇抗性进行了化学诱变改良。获得的突变株 Gf398 在含有 5%（v/v）甲醇的 17%（w/v）甘油培养基中生产了 6.3 g/L 的 GA，而野生型菌株既不生长也不产生 GA。
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