Babu S, Jain N K, Prasad G, Bhattacharya S, Bhattacharya A
New Delhi Tuberculosis Centre, India.
J Med Microbiol. 1990 Aug;32(4):263-9. doi: 10.1099/00222615-32-4-263.
Cell walls from different species of Mycobacterium were purified on a sucrose step gradient. The components derived from these preparations were characterised by sodium dodecyl sulphate-polyacrylamide gel electrophoresis, followed by staining or by Western blotting. Surface-exposed polypeptide molecules were also identified by biotinylation. Many protein and glycoprotein molecules were identified in the cell walls. Some of these molecules were immunogenic in man and experimental animals and showed wide variability from species to species. The data suggest that these molecules could be of significance in the diagnosis and pathophysiology of mycobacterial diseases.
来自不同分枝杆菌属物种的细胞壁在蔗糖阶梯梯度上进行纯化。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳对这些制剂衍生的成分进行表征，随后进行染色或蛋白质免疫印迹分析。还通过生物素化鉴定表面暴露的多肽分子。在细胞壁中鉴定出许多蛋白质和糖蛋白分子。其中一些分子在人和实验动物中具有免疫原性，并且在不同物种之间表现出很大的变异性。数据表明这些分子可能在分枝杆菌疾病的诊断和病理生理学中具有重要意义。
