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利用单克隆抗体对麻风分枝杆菌种特异性及交叉反应性抗原决定簇的定义

Definition of species specific and cross-reactive antigenic determinants of Mycobacterium leprae using monoclonal antibodies.

作者信息

Ivanyi J, Sinha S, Aston R, Cussell D, Keen M, Sengupta U

出版信息

Clin Exp Immunol. 1983 Jun;52(3):528-36.

PMID:6191891
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1536026/
Abstract

Four soluble antigens of Mycobacterium leprae have been identified using 12 murine monoclonal antibodies. Their specificity, taxonomic distribution and molecular nature were analysed by radioimmunoassays and by immunoblotting from polyacrylamide electrophoresis gels. A protein antigen MY1 (12K) reacted with one antibody (ML06) without demonstrable cross-reactivity for any of the other 20 tested species of mycobacteria. Another four antibodies which identified antigen MY2 revealed only a marginal degree of cross-reactivity with three other species of mycobacteria. Two antigens shared by several other mycobacteria species were: MY3 represented by five protein bands (35-70K) and a subtilisin resistant molecule MY4 (40-50K) with two distinct determinants and presumably of polysaccharide nature. The described monoclonal antibodies may represent novel valuable diagnostic reagents as well as tools for the purification of antigens which could be explored towards prophylactic or therapeutic immunization against leprosy.

摘要

利用12种鼠源单克隆抗体鉴定出了麻风分枝杆菌的四种可溶性抗原。通过放射免疫分析以及聚丙烯酰胺电泳凝胶免疫印迹法对它们的特异性、分类分布和分子性质进行了分析。一种蛋白质抗原MY1（12K）与一种抗体（ML06）发生反应，对其他20种受试分枝杆菌物种均未表现出明显的交叉反应。鉴定出抗原MY2的另外四种抗体仅与其他三种分枝杆菌物种表现出轻微程度的交叉反应。其他几种分枝杆菌物种共有的两种抗原为：由五条蛋白带（35 - 70K）代表的MY3和具有两个不同决定簇且可能具有多糖性质的抗枯草杆菌蛋白酶分子MY4（40 - 50K）。所描述的单克隆抗体可能代表新型有价值的诊断试剂以及抗原纯化工具，可用于探索针对麻风病的预防性或治疗性免疫。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e480/1536026/9c4f6a02211a/clinexpimmunol00159-0073-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e480/1536026/9c4f6a02211a/clinexpimmunol00159-0073-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e480/1536026/9c4f6a02211a/clinexpimmunol00159-0073-a.jpg

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