Guadarrama-Avila Tania Marel, Ramírez-Trujillo José Augusto, Rodríguez-Ocampo Thania Gisel, Peña-Chora Guadalupe, Arenas-Sosa Iván, Hernández-Velázquez Víctor Manuel
Laboratorio de Control Biológico, Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Cuernavaca 62209, Mexico.
Laboratorio de Parasitología Vegetal, Centro de Investigaciones Biológicas, Universidad Autónoma del Estado de Morelos, Cuernavaca 62209, Mexico.
Microorganisms. 2023 Oct 16;11(10):2571. doi: 10.3390/microorganisms11102571.
Entomopathogenic nematodes have been used in biological control for some time and are an alternative for the control of insect pests, but during their implementation, situations have arisen that can be improved. These vary with each species and include their production and storage. , an entomopathogenic nematode (EPN), was monitored for its performance when produced in vivo, as well as its development using larvae, using the MC5-2014 strain isolated from soil samples in the municipality of Tepalcingo, Morelos, México. For a study with native strains of EPNs, a wide range of tests must be conducted because the required conditions can be very specific. In vivo production was quantified at initial infective juvenile (IJ) inocula of 50, 100 and 500, and we obtained the same production for the three inocula. The life cycle of the EPNs lasted 12 days, and two generations were observed in which adults were found at days 5 and 9. Both evaluations were performed at a temperature of 27 °C in larvae. In addition, the temperatures of 8, 12, 20 and 24 °C were evaluated for their storage, and we observed that the EPNs can be kept for at least 6 months, maintaining a survival rate of 58.67% and a good infective capacity at a temperature of 12 °C, remaining above 60%.
昆虫病原线虫已被用于生物防治一段时间，是控制害虫的一种替代方法，但在其应用过程中，出现了一些可以改进的情况。这些情况因物种而异，包括它们的生产和储存。对一种昆虫病原线虫（EPN），即从墨西哥莫雷洛斯州特帕尔钦戈市土壤样本中分离出的MC5 - 2014菌株，在其体内生产时的性能以及使用 幼虫进行的发育情况进行了监测。对于一项关于EPN本土菌株的研究，必须进行广泛的测试，因为所需条件可能非常特殊。在初始感染性幼虫（IJ）接种量为50、100和500时对体内生产进行了量化，我们发现这三种接种量的产量相同。EPN的生命周期持续12天，观察到两代，在第5天和第9天发现了成虫。这两项评估均在27°C的温度下对 幼虫进行。此外，对8、12、20和24°C的温度进行了储存评估，我们观察到EPN可以保存至少6个月，在12°C的温度下保持58.67%的存活率和良好的感染能力，存活率保持在60%以上。
