Genomics Research Centre, Griffith Health Institute and School of Medical Science, Griffith University, Gold Coast, QLD, 4222, Australia.
Mol Biol Rep. 2013 Sep;40(9):5483-90. doi: 10.1007/s11033-013-2648-6. Epub 2013 Aug 6.
Here, we investigate the genetic basis of human memory in healthy individuals and the potential role of two polymorphisms, previously implicated in memory function. We have explored aspects of retrospective and prospective memory including semantic, short term, working and long-term memory in conjunction with brain derived neurotrophic factor (BDNF) and tumor necrosis factor-alpha (TNF-α). The memory scores for healthy individuals in the population were obtained for each memory type and the population was genotyped via restriction fragment length polymorphism for the BDNF rs6265 (Val66Met) SNP and via pyrosequencing for the TNF-α rs113325588 SNP. Using univariate ANOVA, a significant association of the BDNF polymorphism with visual and spatial memory retention and a significant association of the TNF-α polymorphism was observed with spatial memory retention. In addition, a significant interactive effect between BDNF and TNF-α polymorphisms was observed in spatial memory retention. In practice visual memory involves spatial information and the two memory systems work together, however our data demonstrate that individuals with the Val/Val BDNF genotype have poorer visual memory but higher spatial memory retention, indicating a level of interaction between TNF-α and BDNF in spatial memory retention. This is the first study to use genetic analysis to determine the interaction between BDNF and TNF-α in relation to memory in normal adults and provides important information regarding the effect of genetic determinants and gene interactions on human memory.
在这里，我们研究了健康个体的人类记忆的遗传基础，以及两个先前与记忆功能相关的多态性的潜在作用。我们已经探讨了回溯和前瞻性记忆的各个方面，包括与脑源性神经营养因子 (BDNF) 和肿瘤坏死因子-α (TNF-α) 相关的语义、短期、工作和长期记忆。通过限制片段长度多态性对 BDNF rs6265 (Val66Met) SNP 进行基因分型，并通过焦磷酸测序对 TNF-α rs113325588 SNP 进行基因分型，获得了人群中每个记忆类型的健康个体的记忆得分。通过单变量方差分析，BDNF 多态性与视觉和空间记忆保留有显著关联，TNF-α 多态性与空间记忆保留有显著关联。此外，BDNF 和 TNF-α 多态性在空间记忆保留中观察到显著的相互作用。实际上，视觉记忆涉及空间信息，两个记忆系统协同工作，但我们的数据表明，BDNF 基因型为 Val/Val 的个体的视觉记忆较差，但空间记忆保留较高，表明 TNF-α 和 BDNF 之间在空间记忆保留方面存在相互作用。这是第一项使用遗传分析来确定 BDNF 和 TNF-α 之间相互作用与正常成年人记忆关系的研究，为遗传决定因素和基因相互作用对人类记忆的影响提供了重要信息。
