Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, China.
Extremophiles. 2013 Sep;17(5):871-9. doi: 10.1007/s00792-013-0570-4. Epub 2013 Aug 6.
It has been suggested that archaea carrying the accA gene, encoding the alpha subunit of the acetyl CoA carboxylase, autotrophically fix CO2 using the 3-hydroxypropionate/4-hydroxybutyrate pathway in low-temperature environments (e.g., soils, oceans). However, little new information has come to light regarding the occurrence of archaeal accA genes in high-temperature ecosystems. In this study, we investigated the abundance and diversity of archaeal accA gene in hot springs in Yunnan Province, China, using DNA- and RNA-based phylogenetic analyses and quantitative polymerase chain reaction. The results showed that archaeal accA genes were present and expressed in the investigated Yunnan hot springs with a wide range of temperatures (66-96 °C) and pH (4.3-9.0). The majority of the amplified archaeal accA gene sequences were affiliated with the ThAOA/HWCG III [thermophilic ammonia-oxidizing archaea (AOA)/hot water crenarchaeotic group III]. The archaeal accA gene abundance was very close to that of AOA amoA gene, encoding the alpha subunit of ammonia monooxygenase. These data suggest that AOA in terrestrial hot springs might acquire energy from ammonia oxidation coupled with CO2 fixation using the 3-hydroxypropionate/4-hydroxybutyrate pathway.
有人提出，携带 accA 基因（编码乙酰辅酶 A 羧化酶的α亚基）的古菌可以在低温环境（例如土壤、海洋）中通过 3-羟基丙酸/4-羟基丁酸途径自养固定 CO2。然而，关于高温生态系统中古菌 accA 基因的发生情况，鲜有新的信息。在这项研究中，我们通过 DNA 和 RNA 系统发育分析和定量聚合酶链反应，调查了中国云南省温泉中古菌 accA 基因的丰度和多样性。结果表明，在所调查的云南温泉中存在并表达古菌 accA 基因，这些温泉的温度范围很广（66-96°C），pH 值范围也很广（4.3-9.0）。扩增出的大多数古菌 accA 基因序列与 ThAOA/HWCG III [嗜热氨氧化古菌（AOA）/热水古菌群 III]有关。古菌 accA 基因的丰度与编码氨单加氧酶α亚基的 AOA amoA 基因非常接近。这些数据表明，陆地温泉中的 AOA 可能通过氨氧化耦合 CO2 固定，利用 3-羟基丙酸/4-羟基丁酸途径获取能量。
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