多环芳烃（PAHs）和重金属混合物对芒鲶鱼肝中乙氧基异吩恶唑酮-O-脱乙基酶（EROD）活性的体外影响研究。
Study on the in vitro effects of the mixtures of polycyclic aromatic hydrocarbons (PAHs) and heavy metals on ethoxyresorufin-O-deethylase (EROD) activity in Mossambica tilapia liver.
机构信息
School of Pharmaceutical Sciences, Xiamen University, Xiamen, 361005, China.
出版信息
Bull Environ Contam Toxicol. 2013 Oct;91(4):460-4. doi: 10.1007/s00128-013-1075-7. Epub 2013 Aug 6.
This paper reports in vitro effects of individual heavy metals (Cd(2+), Cu(2+) and Hg(2+)), and PAHs, including benzo[a]pyrene(BaP), indeno[1,2,3-cd]pyrene (IP) and fluoranthene (FL), and their mixtures on ethoxyresorufin-O-deethylase (EROD) activities using a plate-reader method. The results showed that all three metals inhibited EROD activity, while BaP/IP significantly induced the enzyme. However, FL alone decreased EROD activity. Moreover, co-treatment with BaP/IP and heavy metals inhibited PAH-induced EROD activities, while combined exposure to FL and heavy metals induced FL-inhibited EROD activities.
本文报告了在体外条件下，单一重金属（Cd(2+)、Cu(2+)和 Hg(2+)）和多环芳烃（PAHs），包括苯并[a]芘（BaP）、茚并[1,2,3-cd]芘（IP）和荧蒽（FL），以及它们的混合物对用板读法测定的乙氧基Resorufin-O-脱乙基酶（EROD）活性的影响。结果表明，三种金属均抑制 EROD 活性，而 BaP/IP 则显著诱导该酶。然而，FL 单独降低 EROD 活性。此外，BaP/IP 与重金属联合处理抑制了 PAH 诱导的 EROD 活性，而 FL 与重金属联合暴露则诱导了 FL 抑制的 EROD 活性。
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