体外研究溴系阻燃剂、选定金属及其混合物对莫桑比克罗非鱼肝脏中乙氧基异吩恶唑酮-O-脱乙基酶活性的影响。
In vitro effects of brominated flame retardants, selected metals and their mixtures on ethoxyresorufin-O-deethylase activity in Mossambica tilapia liver.
机构信息
School of Pharmaceutical Sciences, Xiamen University, Xiamen 361102, China; Third Institute of Oceanography, State Oceanic Administration (SOA), Xiamen 361005, China.
Third Institute of Oceanography, State Oceanic Administration (SOA), Xiamen 361005, China.
出版信息
Ecotoxicol Environ Saf. 2018 Oct;161:350-355. doi: 10.1016/j.ecoenv.2018.05.084. Epub 2018 Jun 8.
The in vitro effects of individual brominated flame retardants (BFRs), selected metals, and their binary mixtures on ethoxyresorufin-O-deethylase (EROD) activity were evaluated using a plate-reader method. The BFRs, 2,2',4,4'-tetrabromodiphenyl ether (BDE-47), decabromodiphenyl oxide (BDE-209), hexabromocyclododecane (HBCD), and tetrabromobisphenol A (TBBPA), were tested at doses ranging from 0.1 ng/L to 100 µg/L. Selected metals (Cu, Cd, Hg, and Zn) were screened at doses of 0.1 mg/L to 50 mg/L. The activity of EROD was significantly induced by TBBPA, BDE-209, and Zn, while HBCD, Cu, Cd, and Hg decreased EROD activity. Moreover, following exposure to binary mixtures of metals and BFRs, the EROD activity dose-response curves were similar to those of the metals alone, indicating that EROD activity was governed by the metals.
采用板读法评估了 2,2',4,4'-四溴二苯醚（BDE-47）、十溴联苯醚（BDE-209）、六溴环十二烷（HBCD）和四溴双酚 A（TBBPA）等溴化阻燃剂（BFRs）、以及铜（Cu）、镉（Cd）、汞（Hg）和锌（Zn）等选定金属在体外对乙氧基异吩恶唑-O-脱乙基酶（EROD）活性的影响。BFRs 的测试剂量范围为 0.1ng/L 至 100μg/L，选定金属的测试剂量范围为 0.1mg/L 至 50mg/L。TBBPA、BDE-209 和 Zn 显著诱导 EROD 活性，而 HBCD、Cu、Cd 和 Hg 则降低 EROD 活性。此外，在接触金属和 BFRs 的二元混合物后，EROD 活性剂量反应曲线与单独金属的相似，表明 EROD 活性受金属控制。
Zotero 插件