Haring M A, Rommens C M, Nijkamp H J, Hille J
Free University, Dept. of Genetics, Amsterdam, The Netherlands.
Plant Mol Biol. 1991 Mar;16(3):449-61. doi: 10.1007/BF00023995.
This review compares the activity of the plant transposable elements Ac, Tam3, En/Spm and Mu in heterologous plant species and in their original host. Mutational analysis of the autonomous transposable elements and two-element systems have supplied data that revealed some fundamental properties of the transposition mechanism. Functional parts of Ac and En/Spm were detected by in vitro binding studies of purified transposase protein and have been tested for their importance in the function of these transposable elements in heterologous plant species. Experiments that have been carried out to regulate the activity of the Ac transposable element are in progress and preliminary results have been compiled. Perspectives for manipulated transposable elements in transposon tagging strategies within heterologous plant species are discussed.
本综述比较了植物转座因子Ac、Tam3、En/Spm和Mu在异源植物物种及其原始宿主中的活性。对自主转座因子和双因子系统的突变分析提供了揭示转座机制一些基本特性的数据。通过纯化转座酶蛋白的体外结合研究检测到了Ac和En/Spm的功能部分，并已测试它们在这些转座因子在异源植物物种中的功能中的重要性。为调控Ac转座因子活性而开展的实验正在进行中，初步结果已汇总。文中还讨论了在异源植物物种的转座子标签策略中操纵转座因子的前景。
