基于微堰剪切升力的流体动力学聚焦微通道。
A hydrodynamic focusing microchannel based on micro-weir shear lift force.
机构信息
Department of Engineering Science, National Cheng Kung University, Tainan 70101, Taiwan.
出版信息
Biomicrofluidics. 2012 Aug 6;6(3):34110. doi: 10.1063/1.4739073. Print 2012 Sep.
A novel microflow cytometer is proposed in which the particles are focused in the horizontal and vertical directions by means of the Saffman shear lift force generated within a micro-weir microchannel. The proposed device is fabricated on stress-relieved glass substrates and is characterized both numerically and experimentally using fluorescent particles with diameters of 5 μm and 10 μm, respectively. The numerical results show that the micro-weir structures confine the particle stream to the center of the microchannel without the need for a shear flow. Moreover, the experimental results show that the particles emerging from the micro-weir microchannel pass through the detection region in a one-by-one fashion. The focusing effect of the micro-weir microchannel is quantified by computing the normalized variance of the optical detection signal intensity. It is shown that the focusing performance of the micro-weir structure is equal to 99.76% and 99.57% for the 5-μm and 10-μm beads, respectively. Overall, the results presented in this study confirm that the proposed microcytometer enables the reliable sorting and counting of particles with different diameters.
提出了一种新型微流控芯片，该芯片通过在微堰微通道内产生的 Saffman 剪切升力，在水平和垂直方向上对颗粒进行聚焦。所提出的器件是在弛豫的玻璃衬底上制造的，并用直径分别为 5μm 和 10μm 的荧光颗粒进行了数值和实验特性分析。数值结果表明，微堰结构将颗粒流限制在微通道的中心，而无需剪切流。此外，实验结果表明，从微堰微通道中出来的颗粒逐个通过检测区域。通过计算光学检测信号强度的归一化方差来量化微堰微通道的聚焦效果。结果表明，对于 5μm 和 10μm 的珠子，微堰结构的聚焦性能分别达到了 99.76%和 99.57%。总的来说，本研究的结果证实了所提出的微流控芯片能够可靠地对不同直径的颗粒进行分选和计数。
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