利用隔膜制作润湿性调焦器。
Making a hydrophoretic focuser tunable using a diaphragm.
机构信息
School of Mechanical, Materials and Mechatronic Engineering, University of Wollongong , Wollongong, New South Wales 2522, Australia.
CSIRO Manufacturing Flagship , Private Bag 10, Clayton South, Victoria 3169, Australia.
出版信息
Biomicrofluidics. 2014 Dec 4;8(6):064115. doi: 10.1063/1.4903761. eCollection 2014 Nov.
Microfluidic diagnostic devices often require handling particles or cells with different sizes. In this investigation, a tunable hydrophoretic device was developed which consists of a polydimethylsiloxane (PDMS) slab with hydrophoretic channel, a PDMS diaphragm with pressure channel, and a glass slide. The height of the hydrophoretic channel can be tuned simply and reliably by deforming the elastomeric diaphragm with pressure applied on the pressure channel. This operation allows the device to have a large operating range where different particles and complex biological samples can be processed. The focusing performance of this device was tested using blood cells that varied in shape and size. The hydrophoretic channel had a large cross section which enabled a throughput capability for cell focusing of ∼15 000 cells s(-1), which was more than the conventional hydrophoretic focusing and dielectrophoresis (DEP)-active hydrophoretic methods. This tunable hydrophoretic focuser can potentially be integrated into advanced lab-on-a-chip bioanalysis devices.
微流控诊断设备通常需要处理具有不同尺寸的颗粒或细胞。在这项研究中，开发了一种可调谐的流体动力装置，它由具有流体动力通道的聚二甲基硅氧烷（PDMS）平板、具有压力通道的 PDMS 膜片和玻璃载片组成。通过在压力通道上施加压力使弹性膜片变形，可以简单、可靠地调节流体动力通道的高度。这种操作使该设备具有较大的工作范围，可处理不同的颗粒和复杂的生物样品。使用形状和大小不同的血细胞测试了该装置的聚焦性能。流体动力通道具有较大的横截面，能够实现约 15000 个细胞 s-1 的细胞聚焦通量，这超过了传统的流体动力聚焦和介电泳（DEP）活性流体动力方法。这种可调谐的流体动力聚焦器有可能集成到先进的片上实验室生物分析设备中。
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