微小 RNA-145 通过直接靶向人卵巢癌细胞中的 c-Myc 发挥抑癌基因作用。
MicroRNA-145 function as a cell growth repressor by directly targeting c-Myc in human ovarian cancer.
机构信息
Department of Gynecology and Obstetrics, The Military General Hospital of Beijing PLA, No. 5 Nan Men Cang, Dongcheng District, Beijing 100700, PR China.
出版信息
Technol Cancer Res Treat. 2014 Apr;13(2):161-8. doi: 10.7785/tcrt.2012.500367. Epub 2013 Aug 2.
MiR-145 is reported to be significantly down-regulated in ovarian cancer. This study was aimed at elucidating the roles of miR-145 in regulating the biological behavior of epithelial ovarian cancer (EOC) cells. In this report, we find out that up-regulation of miR-145 in OVCAR-3 and SKOV-3 cells inhibit cell proliferation and promote cell apoptosis. We show that miR-145 directly target the c-Myc 3'-UTR. Moreover, ectopic expression of c-Myc reduces the inhibition of cell proliferation caused by miR-145 transfection. Cell cycle assay showed that up-regulation of miR-145 reduces S phase population, and restoration of c-Myc can rescue this reduction. These findings indicate that miR-145 inhibits cell proliferation and promotes cell apoptosis by targeting c-Myc 3'-UTR. Therefore, the result indicated that miR- 145 could be used as a potential therapeutic target in ovarian cancer.
据报道，miR-145 在卵巢癌中显著下调。本研究旨在阐明 miR-145 在调节上皮性卵巢癌（EOC）细胞生物学行为中的作用。在本报告中，我们发现上调 miR-145 可抑制 OVCAR-3 和 SKOV-3 细胞的增殖并促进细胞凋亡。我们表明 miR-145 可直接靶向 c-Myc 3'-UTR。此外，c-Myc 的异位表达可降低 miR-145 转染引起的细胞增殖抑制。细胞周期分析表明，上调 miR-145 可减少 S 期细胞群体，而 c-Myc 的恢复可挽救这种减少。这些发现表明，miR-145 通过靶向 c-Myc 3'-UTR 抑制细胞增殖并促进细胞凋亡。因此，结果表明 miR-145 可作为卵巢癌的潜在治疗靶点。
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