Yuan Jialing, Wang Kana, Xi Mingrong
Department of Gynecology and Obstetrics, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, China (mainland).
Med Sci Monit. 2016 Feb 24;22:617-24. doi: 10.12659/msm.897288.
BACKGROUND Epithelial ovarian cancer (EOC) is the most lethal malignant gynecological cancer. MicroRNAs (miRNAs) play important roles in the pathogenesis of ovarian cancer. The role of miR-494 in EOC has not been fully investigated. MATERIAL AND METHODS MiR-494 levels in ovarian cancer tissues and cells were tested by qRT-PCR. Cells were transfected with miR-494 mimics or miR-494 ASO by Lipofectamine. Bioinformatics algorithms from TargetScanHuman were used to predict the target genes of miR-494. The c-Myc protein level was assayed by Western blot. The interaction between miR-494 and c-Myc was confirmed by dual luciferase assays. RESULTS MiR-494 showed low levels in EOC tissues and cells. Overexpression of miR-494 inhibited cell growth and migration of EOC cells and vice versa. c-Myc is the targeted gene of miR-494. CONCLUSIONS MiR-494 has an anti-tumor role in EOC via c-Myc.
上皮性卵巢癌（EOC）是最致命的妇科恶性肿瘤。微小RNA（miRNA）在卵巢癌的发病机制中起重要作用。miR-494在EOC中的作用尚未得到充分研究。
采用qRT-PCR检测卵巢癌组织和细胞中miR-494的水平。通过脂质体转染miR-494模拟物或miR-494反义寡核苷酸（ASO）转染细胞。使用TargetScanHuman的生物信息学算法预测miR-494的靶基因。通过蛋白质免疫印迹法检测c-Myc蛋白水平。通过双荧光素酶报告基因检测法确认miR-494与c-Myc之间的相互作用。
miR-494在EOC组织和细胞中呈低水平表达。miR-494的过表达抑制了EOC细胞的生长和迁移，反之亦然。c-Myc是miR-494的靶基因。
miR-494通过c-Myc在EOC中发挥抗肿瘤作用。
