National Agriculture and Food Research Organization, National Institute of Livestock and Grassland Science, Nasushiobara 329-2793, Japan.
Japan Collection of Microorganisms, RIKEN BioResource Center, Tsukuba, Ibaraki 305-0074, Japan.
Int J Syst Evol Microbiol. 2013 Dec;63(Pt 12):4613-4618. doi: 10.1099/ijs.0.053124-0. Epub 2013 Aug 6.
A Gram-reaction-positive, facultatively anaerobic, non-spore-forming and catalase-negative rod-shaped bacterial strain, designated IWT126(T), was isolated from orchardgrass (Dactylis glomerata L.) silage preserved in Hachimantai, Iwate, Japan. The isolate showed growth at 15-45 °C, pH 3.5-7.5 and with 4.0 % (w/v) NaCl. The cell wall peptidoglycan did not contain meso-diaminopimelic acid, and the DNA G+C content was 45.6 mol%. The major cellular fatty acids were C16 : 0 and C19 : 1 cyclo 9,10. Based on 16S rRNA gene sequence similarity, strain IWT126(T) was classified as a member of the genus Lactobacillus and was most closely related to Lactobacillus odoratitofui YIT 11304(T) (98.7 %), Lactobacillus similis JCM 2765(T) (98.5 %), Lactobacillus collinoides JCM 1123(T) (97.6 %), Lactobacillus paracollinoides DSM 15502(T) (97.6 %) and Lactobacillus kimchicus DCY51(T) (96.9 %). Based on sequence analysis of the phenylalanyl-tRNA synthase α-subunit (pheS) gene, strain IWT126(T) was well separated from its phylogenetic neighbours in the genus Lactobacillus. Based on physiological, biochemical and genotypic results, as well as low DNA-DNA relatedness to recognized phylogenetic relatives in the genus Lactobacillus, classification of strain IWT126(T) as a representive of a novel species named Lactobacillus silagei sp. nov. is proposed. The type strain is IWT126(T) ( = JCM 19001(T) = DSM 27022(T)).
一株革兰氏阳性、兼性厌氧、不产芽孢、过氧化氢酶阴性的棒状细菌菌株，被命名为 IWT126(T)，从日本岩手县八幡平保存的果园草（Dactylis glomerata L.）青贮中分离得到。该分离株在 15-45°C、pH 3.5-7.5 和 4.0%（w/v）NaCl 的条件下生长。细胞壁肽聚糖中不含 meso-二氨基庚二酸，DNA G+C 含量为 45.6mol%。主要细胞脂肪酸为 C16:0 和 C19:1 环 9,10。基于 16S rRNA 基因序列相似性，菌株 IWT126(T)被归类为乳杆菌属的一员，与 Lactobacillus odoratitofui YIT 11304(T)（98.7%）、Lactobacillus similis JCM 2765(T)（98.5%）、Lactobacillus collinoides JCM 1123(T)（97.6%）、Lactobacillus paracollinoides DSM 15502(T)（97.6%）和 Lactobacillus kimchicus DCY51(T)（96.9%）最为相似。基于苯丙氨酸-tRNA 合成酶 α 亚基（pheS）基因的序列分析，菌株 IWT126(T)与乳杆菌属中的近缘种在系统发育上分离良好。基于生理、生化和基因型结果，以及与乳杆菌属中公认的系统发育相关的低 DNA-DNA 同源性，建议将菌株 IWT126(T)分类为一种新型物种，命名为青贮乳杆菌（Lactobacillus silagei）sp. nov.。该模式菌株为 IWT126(T)（=JCM 19001(T)=DSM 27022(T)）。
