College of Life Sciences, Northeast Agricultural University, Harbin, 150030, PR China.
College of Food Science, Northeast Agricultural University, Harbin, 150030, PR China.
Curr Microbiol. 2024 Sep 11;81(11):351. doi: 10.1007/s00284-024-03871-x.
Four Gram-stain-positive bacterial strains (designated 475-2, 46-6B, 778-2 and A810-3), isolated from traditional Chinese pickle, were characterized using a polyphasic taxonomic approach. Strain 475-2 was most closely related to the type strain of Lapidilactobacillus achengensis, having 99.9% 16S rRNA gene sequence similarity, 94.1-95.1% average nucleotide identity (ANI) and 57.6% digital DNA-DNA hybridization (dDDH) values. Strain 46-6B was most closely related to the type strain of Secundilactobacillus similis, having 99.8% 16S rRNA gene sequence similarity, 94.3-94.9% ANI and 58.9-59.2% dDDH values. Strains 778-2 and A810-3 were phylogenetically related to the type strains of Streptococcus salivarius, Streptococcus thermophilus and Streptococcus vestibularis, having 99.7-99.9% 16S rRNA gene sequence similarities, 89.1-94.4% ANI and 39.0-55.5% dDDH values. Based upon the data obtained in the present study, three novel species, Lapidilactobacillus salsurivasis sp. nov., Secundilactobacillus muriivasis sp. nov. and Streptococcus parasalivarius sp. nov., are proposed and the type strains are 475-2 (= JCM 36613 = CCTCC AB 2023258 = LMG 33412), 46-6B (= JCM 36612 = CCTCC AB 2023259 = LMG 33411) and 778-2 (= JCM 36614 = CCTCC AB 2023257 = LMG 33413), respectively.
从传统中国泡菜中分离到的 4 株革兰氏阳性细菌菌株（分别命名为 475-2、46-6B、778-2 和 A810-3），采用多相分类法进行了表征。菌株 475-2与阿克氏拉氏乳杆菌的模式菌株最为密切相关，16S rRNA 基因序列相似度为 99.9%，平均核苷酸同一性（ANI）为 94.1-95.1%，数字 DNA-DNA 杂交（dDDH）值为 57.6%。菌株 46-6B 与史密氏拟副乳杆菌的模式菌株最为密切相关，16S rRNA 基因序列相似度为 99.8%，ANI 为 94.3-94.9%，dDDH 值为 58.9-59.2%。菌株 778-2 和 A810-3 在系统发育上与唾液链球菌、嗜热链球菌和前庭链球菌的模式菌株有关，16S rRNA 基因序列相似度为 99.7-99.9%，ANI 为 89.1-94.4%，dDDH 值为 39.0-55.5%。基于本研究获得的数据，提出了三个新种，即拉皮氏乳杆菌 salsurivasis sp. nov.、副乳杆菌 muriivasis sp. nov.和唾液链球菌 parasalivarius sp. nov.，其模式菌株分别为 475-2（=JCM 36613=CCTCC AB 2023258=LMG 33412）、46-6B（=JCM 36612=CCTCC AB 2023259=LMG 33411）和 778-2（=JCM 36614=CCTCC AB 2023257=LMG 33413）。
