Department of Physiology, Superior Institute of Biomedical Sciences, State University of Ceará, Fortaleza, CE, Brazil; Chisthus University Center, Department of Physiology, Fortaleza, CE, Brazil; Department of Biochemistry, Federal University of Ceará, Fortaleza, CE, Brazil.
J Ethnopharmacol. 2013 Oct 7;149(3):694-700. doi: 10.1016/j.jep.2013.07.026. Epub 2013 Aug 3.
Hyptis crenata Pohl ex Benth (Lamiaceae), popularly known as "hortelã-brava" or "hortelã do campo", is widely distributed in the northeast of Brazil. In Brazil, the leaves of this plant have been used in traditional medicine for the treatment of gastrointestinal disturbances, including gastric ulcers. In an attempt to experimentally validate this claimed antiulcerogenic activity, the gastroprotective effects of the essential oil extracted from the leaves of the Hyptis crenata Pohl ex Benth (EOHc) were evaluated in recognized gastric ulcer models in mice.
EOHc was obtained by hydrodistillation and analyzed using gas chromatography-mass spectrometry (GC-MS). Swiss male mice (25-30g) were used for the studies. The gastric ulcers were induced by oral administration of absolute ethanol or indomethacin 45min after oral pretreatment with EOHc, vehicle and positive control drugs. One hour after the ulcerative challenges, the stomachs were removed and the area of the lesions was measured. The volume, pH and total acidity of the gastric secretions were determined using the pylorus ligature model. The gastrointestinal motility was measured using gastric emptying and intestinal transit. The ethanol-induced gastric mucus depletion and lipid peroxidation were also analyzed.
Our findings are as follows: A significant inhibition of gastric lesions induced by absolute ethanol was observed in the mice pre-treated with EOHc, at a dose of 30 and 100 and 300mg/kg (5.56±1.51, 2.88±0.82 and 1.71±0.54mm(2), respectively) compared to control group (118.03±35.4mm(2)). Also, EOHc (300mg/kg) produced a gastroprotective effect against the gastric lesions induced by indomethacin (16.07±4.68mm(2)) compared to control group (38.64±6.1mm(2)). EOHc pretreatment produced a reduction in the ethanol-induced lipid peroxidation from 3.9±0.22 to 2.4±0.1μmol/mg tissue (EOHc-300mg/kg and control group, respectively). We also observed that EOHc pretreatment decreased the gastric emptying, but did not alter the intestinal transit ratio, ethanol-induced depletion of the gastric wall mucus or secretion parameters (volume, pH and [H(+)]).
Our data indicate that EOHc exerts a gastroprotective effect, indicated by its significant inhibition of gastric lesions in ethanol- and indomethacin-induced ulcer models, which may be associated with its accelerating effect on gastric emptying and reduction in oxidative damages. Our data suggest a potential therapeutic application for EOHc in the treatment of gastric ulcers.
Hyptis crenata Pohl ex Benth（唇形科），俗称“辣薄荷”或“田间薄荷”，广泛分布于巴西东北部。在巴西，这种植物的叶子被用于传统医学治疗胃肠道紊乱，包括胃溃疡。为了实验验证这种声称的抗溃疡活性，我们评估了从 Hyptis crenata Pohl ex Benth（EHOC）叶子中提取的精油（EOHC）在公认的小鼠胃溃疡模型中的胃保护作用。
EHOC 通过水蒸馏获得，并使用气相色谱-质谱联用仪（GC-MS）进行分析。我们使用 25-30g 的瑞士雄性小鼠进行研究。在口服预处理 EOHC、载体和阳性对照药物 45 分钟后，通过口服给予绝对乙醇或吲哚美辛诱导胃溃疡。在溃疡形成挑战后 1 小时，取出胃并测量病变面积。通过幽门结扎模型测定胃分泌物的体积、pH 值和总酸度。使用胃排空和肠转运来测量胃肠道动力。还分析了乙醇诱导的胃粘液耗竭和脂质过氧化。
我们的研究结果如下：与对照组（118.03±35.4mm(2)）相比，用 EOHC（30 和 100 和 300mg/kg）预处理的小鼠胃中由绝对乙醇诱导的溃疡明显减少（分别为 5.56±1.51、2.88±0.82 和 1.71±0.54mm(2)）。此外，EOHC（300mg/kg）对吲哚美辛诱导的胃溃疡也有保护作用（与对照组相比为 16.07±4.68mm(2)）。EOHC 预处理可将乙醇诱导的脂质过氧化从 3.9±0.22 降低至 2.4±0.1μmol/mg 组织（EOHC-300mg/kg 和对照组）。我们还观察到，EOHC 预处理可减少胃排空，但不改变肠转运率、乙醇诱导的胃壁粘液耗竭或分泌参数（体积、pH 值和[H(+)）。
我们的数据表明，EOHC 通过其对乙醇和吲哚美辛诱导的溃疡模型中胃损伤的显著抑制作用发挥胃保护作用，这可能与其对胃排空的加速作用和氧化损伤的减少有关。我们的数据表明，EOHC 在治疗胃溃疡方面具有潜在的治疗应用。
