Laboratório de Fisiologia Experimental, Instituto Superior de Ciências Biomédicas, Universidade Estadual do Ceará, Campus do Itaperi, Fortaleza, CE, Brasil.
Programa de Pós-Graduação em Bioquímica, Universidade Federal do Ceará, Fortaleza, CE, Brasil.
Braz J Med Biol Res. 2021 Jan 25;54(3):e9422. doi: 10.1590/1414-431X20209422. eCollection 2021.
Hyptis crenata, commonly known as "salva-do-Marajó", "hortelã-do-campo", and "hortelãzinha", is used in folk medicine in Northeast Brazil as tea or infusion to treat inflammatory diseases. Due to the pharmacological efficacy and the low toxicity of the essential oil of Hyptis crenata (EOHc), we decided to investigate the EOHc antiedematogenic effect in experimental models of inflammation. EOHc was administrated orally at doses of 10-300 mg/kg to male Swiss albino mice. Paw edema was induced by subcutaneous injection in the right hind paw of inflammatory stimuli (carrageenan, dextran, histamine, serotonin, and bradykinin) 60 min after administration of EOHc. EOHc significantly inhibited the induced edema. The inhibitory effect of EOHc on dextran-induced edema extended throughout the experimental time. For the 30, 100, and 300 mg/kg doses of EOHc, the inhibition was of 40.28±1.70, 51.18±2.69, and 59.24±2.13%, respectively. The EOHc inhibitory effect on carrageenan-induced edema started at 10 mg/kg at the second hour (h) and was maintained throughout the observation period. At 30, 100, and 300 mg/kg doses the inhibition started earlier, from 30 min. At the edema peak of 180 min, 56, 76, and 82% inhibition was observed for 30, 100, and 300 mg/kg doses, respectively. Additionally, the effect of EOHc on carrageenan-induced paw edema was influenced by the time of administration. The EOHc also inhibited myeloperoxidase activity. In conclusion, the EOHc showed a potent effect, both preventing and reversing the edema, consistent with its anti-inflammatory use in folk medicine.
皱叶酸模，俗称“马拉若岛救星”、“田间薄荷”和“小薄荷”，在巴西东北部的民间医学中被用作茶或浸剂来治疗炎症性疾病。由于皱叶酸模精油（HEOc）具有药理学功效和低毒性，我们决定研究其在炎症实验模型中的抗水肿作用。HEOc 以 10-300mg/kg 的剂量口服给予雄性瑞士白化小鼠。在给予 HEOc 后 60 分钟，通过在右后爪皮下注射炎症刺激物（角叉菜胶、葡聚糖、组胺、血清素和缓激肽）诱导爪水肿。HEOc 显著抑制诱导的水肿。HEOc 对葡聚糖诱导的水肿的抑制作用在整个实验过程中持续存在。对于 30、100 和 300mg/kg 剂量的 HEOc，抑制率分别为 40.28±1.70%、51.18±2.69%和 59.24±2.13%。HEOc 对角叉菜胶诱导的水肿的抑制作用从第 2 小时（h）开始在 10mg/kg 剂量下开始，并在整个观察期内持续。在 30、100 和 300mg/kg 剂量下，抑制作用更早，从 30min 开始。在 180min 的水肿峰值时，观察到 30、100 和 300mg/kg 剂量的抑制率分别为 56%、76%和 82%。此外，HEOc 对角叉菜胶诱导的爪水肿的作用受给药时间的影响。HEOc 还抑制髓过氧化物酶活性。总之，HEOc 表现出强大的作用，既能预防又能逆转水肿，与民间医学中抗炎的应用一致。
