Department of Pharmaceutical Sciences, Federal University of Pernambuco, 50740-521 Recife, PE, Brazil.
J Ethnopharmacol. 2011 Sep 1;137(1):886-92. doi: 10.1016/j.jep.2011.07.005. Epub 2011 Jul 8.
Hyptis martiusii (Lamiaceae), popularly known as "cidreira-do-mato" or "cidreira-brava", grows in abundance in the Northeast region of Brazil, where its leaves are traditionally used in folk medicine in the treatment of intestinal and stomach diseases. The aim of this study was to characterize the chemical constituents and to evaluate the anti-ulcerogenic activity of the essential oil of the leaves of Hyptis martiusii (EOHM) in in vivo models of experimental ulcers in rodents.
EOHM was obtained by hydrodistillation and analyzed by gas chromatography-mass spectrometry (GC-MS). Acute gastric ulcer was induced using absolute ethanol, HCl/ethanol and indomethacin. The volume, pH and total acidity of gastric secretion were determined by the pyloric ligature method and gastrointestinal motility using gastric emptying and intestinal transit.
Chemical analysis revealed the presence of 24 components that account for 92.13% of the essential oil of dried leaves, indicating the occurrence of mono and sesquiterpenes. Oral administration of EOHM (100, 200 and 400mg/kg) inhibited ethanol-, HCl/ethanol- and indomethacin-induced ulcers. In the case of pylorus ligature, the oil reduced the volume of gastric juices and total acidity, and increased gastric pH. The EOHM reduced the rate of gastric emptying with only the highest doses, but did not show any effect on intestinal transit at any of the three doses.
The results indicate that the essential oil of leaves of Hyptis martiusii has an antiulcerogenic activity, as evidenced by its significant inhibition of the formation of ulcers in various models. This effect could be related to an increase of gastric mucosal defensive factors. Further pharmacological studies are being undertaken in order to provide more precise elucidation of the action mechanism involved in this activity.
Hyptis martiusii（唇形科），俗称“cidreira-do-mato”或“cidreira-brava”，在巴西东北部大量生长，其叶子在传统医学中被用于治疗肠道和胃部疾病。本研究旨在对其叶子的精油（EOHM）的化学成分进行表征，并评估其在实验性溃疡的体内模型中的抗溃疡活性。
EOHM 通过水蒸馏法获得，并通过气相色谱-质谱联用（GC-MS）进行分析。使用无水乙醇、HCl/乙醇和吲哚美辛诱导急性胃溃疡。通过幽门结扎法测定胃分泌的体积、pH 值和总酸度，通过胃排空和肠传输测定胃肠道蠕动。
化学分析表明，干叶精油中含有 24 种成分，占 92.13%，表明存在单萜和倍半萜。EOHM（100、200 和 400mg/kg）的口服给药可抑制乙醇、HCl/乙醇和吲哚美辛诱导的溃疡。在幽门结扎的情况下，该油减少了胃液的体积和总酸度，并增加了胃 pH 值。该油降低了胃排空率，只有最高剂量才会出现，但在三个剂量下对肠传输均无影响。
结果表明，Hyptis martiusii 的叶子精油具有抗溃疡活性，其在各种模型中对溃疡形成的显著抑制作用证明了这一点。这种作用可能与胃黏膜防御因子的增加有关。正在进行进一步的药理学研究，以更精确地阐明该活性涉及的作用机制。
