TTan H S, Beiser C
J Pharm Sci. 1975 Jul;64(7):1207-10. doi: 10.1002/jps.2600640718.
A fluorometric procedure for procainamide hydrochloride was developed, and it offers improvements in ease, speed, and sensitivity over the official method. The new procedure is based on the reaction with fluorescamine in aqueous medium at pH 7.5 to form a fluorophore, with activation and emission wavelengths of 400 and 485 nm, respectively. The fluorescence is linear (r = 0.999) over the 0. 04-1 mug/ml concentration range and is stable for at least 2 hr. Recovery data appeared to be accurate, quantitative, and reproducible. The overall recovery was 99.8% with a standard deviation of +/-1.14 (n = 5). The method was successfully applied to commercially available dosage forms.
开发了一种盐酸普鲁卡因胺的荧光测定法，与法定方法相比，该方法在简便性、速度和灵敏度方面有所改进。新方法基于在pH 7.5的水性介质中与荧光胺反应形成荧光团，其激发波长和发射波长分别为400nm和485nm。在0.04 - 1μg/ml浓度范围内荧光呈线性（r = 0.999），且至少稳定2小时。回收率数据准确、定量且可重现。总体回收率为99.8%，标准差为±1.14（n = 5）。该方法成功应用于市售剂型。
