Whitaker J E, Hoyt A M
J Pharm Sci. 1984 Aug;73(8):1184-5. doi: 10.1002/jps.2600730845.
A simple, rapid colorimetric method is described which determines procainamide in commercially available, injectable solutions. It is based on complexing the drug with cupric ion and then measuring the absorption at 380 nm. The reaction is fast and the metal-drug ratio in the complex is 1:1. The pH optimum for maximum reaction is 4.0-4.5 which is maintained by an acetate buffer. Linear standard curves were obtained from 1.52 to 6.06 mg/mL. Analysis of 29 commercial samples resulted in an average of 97.3% of the label claim.
本文描述了一种简单、快速的比色法，用于测定市售注射用溶液中的普鲁卡因胺。该方法基于药物与铜离子络合，然后测量380nm处的吸光度。反应快速，络合物中金属与药物的比例为1:1。最大反应的最佳pH值为4.0-4.5，由醋酸盐缓冲液维持。线性标准曲线在1.52至6.06mg/mL范围内获得。对29个商业样品的分析结果显示，平均含量为标签标示量的97.3%。
