日粮精氨酸和谷氨酰胺对缓解呕吐毒素胁迫及免疫相关细胞因子对生长猪造成损伤的影响。
Effects of dietary arginine and glutamine on alleviating the impairment induced by deoxynivalenol stress and immune relevant cytokines in growing pigs.
机构信息
Hunan Engineering and Research Center of Animal and Poultry Science, and Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan, China.
出版信息
PLoS One. 2013 Jul 29;8(7):e69502. doi: 10.1371/journal.pone.0069502. Print 2013.
Deoxynivalenol (DON) is a mycotoxin that reduces feed intake and animal performance, especially in swine. Arginine and glutamine play important roles in swine nutrition. The objective of this study was to determine the effects of dietary supplementation with arginine and glutamine on both the impairment induced by DON stress and immune relevant cytokines in growing pigs. A total of forty 60-d-old healthy growing pigs with a mean body weight of 16.28±1.54 kg were randomly divided into 5 groups, and assigned to 3 amino acid treatments fed 1.0% arginine (Arg), 1.0% glutamine (Gln) and 0.5% Arg+0.5% Gln, respectively, plus a toxin control and a non-toxin control. Pigs in the 3 amino acid treatments were fed the corresponding amino acids, and those in non-toxin control and toxin control were fed commercial diet with 1.64% Alanine as isonitrogenous control for 7 days. The toxin control and amino acid treatments were then challenged by feeding DON-contaminated diet with a final DON concentration of 6 mg/kg of diet for 21 days. No significant differences were observed between toxin control and the amino acid groups with regard to the average daily gain (ADG), although the values for average daily feed intake (ADFI) in the amino acid groups were significantly higher than that in toxin control (P<0.01). The relative liver weight in toxin control was significantly greater than those in non-toxin control, arginine and Arg+Glu groups (P<0.01), but there were no significant differences in other organs. With regard to serum biochemistry, the values of BUN, ALP, ALT and AST in the amino acid groups were lower than those in toxin control. IGF1, GH and SOD in the amino acid groups were significantly higher than those in toxin control (P<0.01). The IL-2 and TNFα values in the amino acid groups were similar to those in non-toxin control, and significantly lower than those in toxin control (P<0.01). These results showed the effects of dietary supplementation with arginine and glutamine on alleviating the impairment induced by DON stress and immune relevant cytokines in growing pigs.
脱氧雪腐镰刀菌烯醇（DON）是一种霉菌毒素，会降低饲料摄入量和动物生产性能，尤其是在猪中。精氨酸和谷氨酰胺在猪的营养中起着重要作用。本研究的目的是确定日粮补充精氨酸和谷氨酰胺对 DON 应激诱导的损伤和免疫相关细胞因子的影响。总共 40 头 60 日龄、体重平均为 16.28±1.54kg 的健康生长猪被随机分为 5 组，分别饲喂 1.0%精氨酸（Arg）、1.0%谷氨酰胺（Gln）和 0.5%Arg+0.5%Gln，以及毒素对照和非毒素对照。在 3 种氨基酸处理组中，猪被饲喂相应的氨基酸，在非毒素对照和毒素对照中，猪被饲喂含有 1.64%丙氨酸的商业日粮，作为等氮对照，共 7 天。然后，毒素对照和氨基酸处理组通过饲喂终浓度为 6mg/kg 日粮的 DON 污染日粮进行为期 21 天的挑战。在平均日增重（ADG）方面，毒素对照和氨基酸组之间没有观察到显著差异，尽管氨基酸组的平均日采食量（ADFI）显著高于毒素对照（P<0.01）。毒素对照的相对肝重显著大于非毒素对照、精氨酸和 Arg+Glu 组（P<0.01），但其他器官没有显著差异。在血清生化方面，氨基酸组的 BUN、ALP、ALT 和 AST 值低于毒素对照。氨基酸组的 IGF1、GH 和 SOD 显著高于毒素对照（P<0.01）。氨基酸组的 IL-2 和 TNFα 值与非毒素对照相似，显著低于毒素对照（P<0.01）。这些结果表明，日粮补充精氨酸和谷氨酰胺可缓解 DON 应激对生长猪的损伤和免疫相关细胞因子的影响。
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