Postdoctoral Research Base, Henan Collaborative Innovation Center of Modern Biological Breeding, Henan Institute of Science and Technology, Xinxiang 453003, China.
State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai'an 271018, China.
Int J Mol Sci. 2022 Oct 10;23(19):12040. doi: 10.3390/ijms231912040.
CIPK (calcineurin B-like-interacting protein kinase) is a kind of serine/threonine protein kinase widely existing in plants, and it plays an important role in plant growth and development and stress response. To better understand the biological functions of the gene in upland cotton, the coding sequence (CDS) of the gene was cloned in upland cotton, and its protein sequence, evolutionary relationship, subcellular localization, expression pattern and -acting elements in the promoter region were analyzed. Our results showed that the full-length CDS of was 1368 bp, encoding a protein with 455 amino acids. The molecular weight and isoelectric point of this protein were 50.83 KDa and 8.94, respectively. The GhCIPK23 protein contained a conserved N-terminal protein kinase domain and C-terminal regulatory domain of the gene family member. Phylogenetic tree analysis demonstrated that GhCIPK23 had a close relationship with AtCIPK23, followed by OsCIPK23, and belonged to Group A with AtCIPK23 and OsCIPK23. The subcellular localization experiment indicated that GhCIPK23 was located in the plasma membrane. Tissue expression analysis showed that had the highest expression in petals, followed by sepals, and the lowest in fibers. Stress expression analysis showed that the expression of the gene was in response to drought, salt, low-temperature and exogenous abscisic acid (ABA) treatment, and had different expression patterns under different stress conditions. Further -acting elements analysis showed that the promoter region had -acting elements in response to abiotic stress, phytohormones and light. These results established a foundation for understanding the function of and breeding varieties with high-stress tolerance in cotton.
钙调神经磷酸酶 B 样相互作用蛋白激酶（CIPK）是一种广泛存在于植物中的丝氨酸/苏氨酸蛋白激酶，它在植物生长发育和应激响应中发挥着重要作用。为了更好地理解该基因在陆地棉中的生物学功能，我们克隆了陆地棉中的基因编码序列（CDS），并对其蛋白质序列、进化关系、亚细胞定位、表达模式以及启动子区中的顺式作用元件进行了分析。研究结果表明，基因的全长 CDS 为 1368 bp，编码一个含有 455 个氨基酸的蛋白质。该蛋白的分子量和等电点分别为 50.83 KDa 和 8.94。GhCIPK23 蛋白含有一个保守的 N 端蛋白激酶结构域和 C 端基因家族成员的调节结构域。系统进化树分析表明，GhCIPK23 与 AtCIPK23 关系密切，其次是 OsCIPK23，与 AtCIPK23 和 OsCIPK23 一起归属于 A 组。亚细胞定位实验表明，GhCIPK23 定位于质膜上。组织表达分析表明，基因在花瓣中表达量最高，其次是萼片，在纤维中表达量最低。胁迫表达分析表明，基因的表达对干旱、盐、低温和外源脱落酸（ABA）处理有响应，并在不同胁迫条件下表现出不同的表达模式。进一步的顺式作用元件分析表明，启动子区存在对非生物胁迫、植物激素和光照有响应的顺式作用元件。这些结果为理解基因的功能以及在棉花中培育具有高抗逆性的品种奠定了基础。
