The Center of Diagnosis and Treatment for Joint Disease, Drum Tower Hospital Affiliated to Medical School of Nanjing University, Jiangsu, PR China.
PLoS One. 2013 Jul 26;8(7):e70033. doi: 10.1371/journal.pone.0070033. Print 2013.
Deep vein thrombosis is one of the common complications of orthopedic surgery. Studies indicated that genetic factors played a considerable role in the pathogenesis of deep vein thrombosis. Endothelial nitric oxide synthase which encoded by nitric oxide synthase 3 (NOS3), can generate nitric oxide in endothelial cells. As a predominant regulator for vascular homeostasis, nitric oxide might be involved in the pathogenesis of thrombosis. It had been proved that the NOS3 polymorphism (rs1799983) was associated with the development of cardiovascular diseases. Our objective was to evaluate the association between the NOS3 polymorphism (rs1799983) and deep vein thrombosis after orthopedic surgery in Chinese Han population. The polymorphism was genotyped in 224 subjects with deep vein thrombosis after orthopedic surgery and 580 controls. Allele and genotype frequencies were compared between subjects with deep vein thrombosis and control subjects. The allele and genotype frequencies of the NOS3 polymorphism (rs1799983) were significantly different between subjects with deep vein thrombosis and control subjects. There were also significant differences when the subjects were stratified by gender, surgery type and hypertension status. These findings suggested that the NOS3 polymorphism (rs1799983) was associated with susceptibility to the deep vein thrombosis after orthopedic surgery in Chinese Han population, and NOS3 might play a role in the development of deep vein thrombosis after orthopedic surgery.
深静脉血栓形成是骨科手术常见的并发症之一。研究表明，遗传因素在深静脉血栓形成的发病机制中起重要作用。内皮型一氧化氮合酶（NOS3）编码的内皮型一氧化氮合酶可在内皮细胞中产生一氧化氮。作为血管稳态的主要调节因子，一氧化氮可能参与血栓形成的发病机制。已经证明 NOS3 多态性（rs1799983）与心血管疾病的发生有关。我们的目的是评估 NOS3 多态性（rs1799983）与汉族人群骨科手术后深静脉血栓形成的关系。在 224 例骨科手术后深静脉血栓形成患者和 580 例对照者中对该多态性进行了基因分型。比较深静脉血栓形成患者与对照组之间的等位基因和基因型频率。NOS3 多态性（rs1799983）的等位基因和基因型频率在深静脉血栓形成患者与对照组之间存在显著差异。按性别、手术类型和高血压状态分层时，也存在显著差异。这些发现表明，NOS3 多态性（rs1799983）与汉族人群骨科手术后深静脉血栓形成的易感性相关，NOS3 可能在骨科手术后深静脉血栓形成的发生中起作用。
