Neto Abel Barbosa Lira, Farias Myrla C O, Vasconcelos Nancy B R, Xavier Antonio F, Assunção Monica L, Ferreira Haroldo S
Faculty of Nutrition, Federal University of Alagoas, Maceió, AL, Brazil.
Postgraduate Program in Health Sciences of the Institute of Biological and Health Sciences, Federal University of Alagoas, Maceió, AL, Brazil.
Arch Med Sci Atheroscler Dis. 2019 Apr 18;4:e63-e73. doi: 10.5114/amsad.2019.84539. eCollection 2019.
Hypertension is one of the most prevalent diseases in the world, accounting for millions of deaths each year. The reduction in the concentration of nitric oxide (NO) produced by the catalysis of endothelial nitric oxide synthase (eNOS) is associated with higher blood pressure (BP) levels. This reduction might be because of genetic polymorphisms. This study investigated the prevalence of the eNOS gene G894T polymorphism in women from northeast Brazil and its association with hypertension.
This cross-sectional study included 810 women (aged 19-49 years). Sociodemographic, health, anthropometric, and BP data were collected. Hypertension was defined according to the following criteria: systolic BP ≥ 140 mm Hg, diastolic BP ≥ 90 mm Hg, the regular use of antihypertensive medication, or some combination thereof. Epithelial cells from the cheek mucosa were obtained for DNA extraction. Genotyping was performed via real-time PCR. The measure of association was the prevalence ratio (PR) and its 95% CI as calculated via Poisson regression.
The frequencies of the , , and genotypes were 57.1%, 35.7%, and 7.2%, respectively. For each of these genotypes, the prevalence of hypertension in women was 17.9%, 23.6%, and 34.4%, respectively. Relative to the genotype, the PRs after adjusting for cofounding factors were 1.24 (95% CI: 0.95-1.61, = 0.11) for and 1.76 (95% CI: 1.16-2.67, < 0.01) for .
The allele of the G894T polymorphisms is associated with hypertension in women. This may have implications for prevention and treatment.
高血压是世界上最常见的疾病之一，每年导致数百万人死亡。内皮型一氧化氮合酶（eNOS）催化产生的一氧化氮（NO）浓度降低与较高的血压（BP）水平相关。这种降低可能是由于基因多态性。本研究调查了巴西东北部女性中eNOS基因G894T多态性的患病率及其与高血压的关联。
这项横断面研究纳入了810名女性（年龄在19 - 49岁之间）。收集了社会人口统计学、健康、人体测量和血压数据。高血压根据以下标准定义：收缩压≥140 mmHg，舒张压≥90 mmHg；定期使用抗高血压药物；或上述情况的某种组合。采集颊黏膜上皮细胞用于DNA提取。通过实时PCR进行基因分型。关联度的测量指标是患病率比（PR）及其通过泊松回归计算的95%置信区间（CI）。
GG、GT和TT基因型的频率分别为57.1%、35.7%和7.2%。对于每种基因型，女性高血压的患病率分别为17.9%、23.6%和34.4%。相对于GG基因型，在调整混杂因素后，GT基因型的PR为1.24（95% CI：0.95 - 1.61，P = 0.11），TT基因型的PR为1.76（95% CI：1.16 - 2.67，P < 0.01）。
G894T多态性的T等位基因与女性高血压相关。这可能对预防和治疗有影响。
