Aggarwal S, Tandon C, Forouzandeh M, Singla S K, Kiran R, Jethi R K
Department of Biochemistry, Panjab University, Chandigarh-160 014, India.
Indian J Biochem Biophys. 2005 Apr;42(2):113-7.
The role of biomolecule(s) from renal stone matrix in urolithiasis was investigated. The ability of a particular fraction (> 10 kDa fraction) isolated from the EDTA extract of powdered human renal stones to influence calcium oxalate monohydrate (COM) crystal growth was studied. The most potent inhibitor of COM crystal growth obtained from > 10 kDa fraction was purified by various chromatographic techniques and SDS-PAGE, etc. and was found to have a molecular mass of 36 kDa. The urine and serum samples obtained from normal persons were found to be more potent in inhibiting the growth of COM crystals as compared to the kidney-stone patients. Polyclonal antibodies were raised against this inhibitor and were employed to determine the concentration of 36 kDa inhibitor in urine and serum samples of normal persons and kidney-stone patients.
研究了来自肾结石基质的生物分子在尿路结石形成中的作用。研究了从人肾结石粉末的EDTA提取物中分离出的特定组分（>10 kDa组分）影响一水草酸钙（COM）晶体生长的能力。通过各种色谱技术和SDS-PAGE等方法对从>10 kDa组分中获得的最有效的COM晶体生长抑制剂进行了纯化，发现其分子量为36 kDa。结果发现，与肾结石患者相比，正常人的尿液和血清样本在抑制COM晶体生长方面更有效。制备了针对该抑制剂的多克隆抗体，并用于测定正常人和肾结石患者尿液和血清样本中36 kDa抑制剂的浓度。
