Gautam M K, Ghatule R R, Singh A, Purohit V, Gangwar M, Kumar Mohan, Goel R K
Department of Pharmacology, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221 005, India.
Indian J Exp Biol. 2013 Feb;51(2):157-64.
Graded doses of 50% ethanolic extract of dried fruit pulp of Aegle marmelos (AME) (100, 200 and 400 mg/kg) daily for 14 days in acetic acid (AA)-induced colitis in rats showed 200 mg/kg of AME as an optimal effective dose against AA-induced colonic damage score and weight. This dose (200 mg/kg; po) was further studied in AA-induced colitis for its effects on various physical (mucous/blood in stool, food and water intake and body weight changes), histology, antibacterial activity and biochemical parameters like free radicals (nitric oxide and lipid peroxidation), antioxidants (superoxide dismutase, catalase and reduced glutathione) and myeloperoxidase (acute-inflammatory marker) activities in rat colonic tissue. AME decreased colonic mucosal damage and inflammation (macroscopic and microscopic), mucous/bloody diarrhea, fecal frequency and increased body weight affected in AA-induced colitis. AME showed significant antibacterial activity and enhanced the antioxidants but decreased free radicals and myeloperoxidase activities thereby decreasing tissue damage and inflammation and thus, affording ulcer healing. The above effects of A. marmelos authenticated its use in indigenous system of Medicine.
在大鼠乙酸诱导的结肠炎模型中，连续14天每日给予不同剂量（100、200和400毫克/千克）的印度枳椇干燥果肉50%乙醇提取物（AME），结果显示200毫克/千克的AME是对抗乙酸诱导的结肠损伤评分和体重的最佳有效剂量。该剂量（200毫克/千克；口服）在乙酸诱导的结肠炎模型中进一步研究其对各种生理指标（粪便中的黏液/血液、食物和水摄入量以及体重变化）、组织学、抗菌活性以及大鼠结肠组织中自由基（一氧化氮和脂质过氧化）、抗氧化剂（超氧化物歧化酶、过氧化氢酶和还原型谷胱甘肽）和髓过氧化物酶（急性炎症标志物）活性等生化参数的影响。AME减轻了结肠黏膜损伤和炎症（宏观和微观）、黏液/血性腹泻、粪便频率，并增加了乙酸诱导的结肠炎所影响的体重。AME显示出显著的抗菌活性，增强了抗氧化剂，但降低了自由基和髓过氧化物酶活性，从而减少组织损伤和炎症，促进溃疡愈合。印度枳椇的上述作用证实了其在传统医学中的应用。
