Laboratoire Matière et Systèmes Complexes, UMR 7057, CNRS and Université Paris Diderot , France.
ACS Nano. 2013 Sep 24;7(9):7500-12. doi: 10.1021/nn401095p. Epub 2013 Aug 20.
Recent advances in cell therapy and tissue engineering opened new windows for regenerative medicine, but still necessitate innovative noninvasive imaging technologies. We demonstrate that high-resolution magnetic resonance imaging (MRI) allows combining cellular-scale resolution with the ability to detect two cell types simultaneously at any tissue depth. Two contrast agents, based on iron oxide and gadolinium oxide rigid nanoplatforms, were used to "tattoo" endothelial cells and stem cells, respectively, with no impact on cell functions, including their capacity for differentiation. The labeled cells' contrast properties were optimized for simultaneous MRI detection: endothelial cells and stem cells seeded together in a polysaccharide-based scaffold material for tissue engineering appeared respectively in black and white and could be tracked, at the cellular level, both in vitro and in vivo. In addition, endothelial cells labeled with iron oxide nanoparticles could be remotely manipulated by applying a magnetic field, allowing the creation of vessel substitutes with in-depth detection of individual cellular components.
细胞治疗和组织工程的最新进展为再生医学开辟了新的窗口，但仍需要创新的非侵入性成像技术。我们证明，高分辨率磁共振成像（MRI）允许将细胞尺度分辨率与在任何组织深度同时检测两种细胞类型的能力相结合。两种造影剂，基于氧化铁和钆氧化物刚性纳米平台，分别用于“纹身”内皮细胞和干细胞，对细胞功能没有影响，包括分化能力。标记细胞的对比特性经过优化，可用于同时进行 MRI 检测：在组织工程中一起种植在多糖基支架材料中的内皮细胞和干细胞分别呈现黑色和白色，可以在体外和体内进行细胞水平的跟踪。此外，用氧化铁纳米粒子标记的内皮细胞可以通过施加磁场进行远程操作，允许用个体细胞成分的深度检测来创建血管替代品。
