一氧化氮、氯离子、醋酸根离子和二甘醇对氧化铈纳米颗粒的形态、结构、抗糖尿病特性及细胞活力的影响。

Effects of NO, Cl, and CHCOO anions and diethylene glycol on the morphological, structural, antidiabetic, and cell viability properties of CeO nanoparticles.

作者信息

Matussin Shaidatul Najihah, Khan Fazlurrahman, Chandika Pathum, Harunsani Mohammad Hilni, Ahmad Norhayati, Kim Young-Mog, Jung Won-Kyo, Khan Mohammad Mansoob

机构信息

Chemical Sciences, Faculty of Science, Universiti Brunei Darussalam Jalan Tungku Link Gadong BE 1410 Brunei Darussalam

Marine Integrated Biomedical Technology Center, The National Key Research Institutes in Universities, Pukyong National University Busan 48513 Republic of Korea.

出版信息

RSC Adv. 2023 May 22;13(23):15421-15436. doi: 10.1039/d3ra02474a.

DOI:10.1039/d3ra02474a

PMID:37223416

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10201339/

Abstract

Cerium oxide (CeO) nanoparticles (NPs) were synthesized using a modified conventional polyol method. The ratio of diethylene glycol (DEG) and water in the synthesis was varied, and three different cerium precursor salts (Ce(NO), CeCl, and Ce(CHCOO)) were used. The structure, size, and morphology of the synthesized CeO NPs were studied. An average crystallite size of 13 to 33 nm was obtained from the XRD analysis. Spherical and elongated morphologies of the synthesized CeO NPs were acquired. Average particle sizes in the range of 16-36 nm were obtained by varying different ratios of DEG and water. The presence of DEG molecules on the surface of CeO NPs was confirmed using FTIR. Synthesized CeO NPs were used to study the antidiabetic and cell viability (cell cytotoxicity) properties. Antidiabetic studies were carried out using α-glucosidase enzymes inhibition activity. CeO synthesized using Ce(NO) and CeCl precursors showed approximately 40.0% α-glucosidase enzyme inhibition activity, while CeO synthesized using Ce(CHCOO) showed the lowest α-glucosidase enzyme inhibition activity. Cell viability properties of CeO NPs were investigated using an cytotoxicity test. CeO NPs prepared using Ce(NO) and CeCl were non-toxic at lower concentrations, while CeO NPs prepared using Ce(CHCOO) were non-toxic at all concentrations. Therefore, polyol-mediated synthesized CeO NPs showed quite good α-glucosidase inhibition activity and biocompatibility.

摘要

采用改进的传统多元醇法合成了氧化铈（CeO）纳米颗粒（NPs）。合成过程中改变了二甘醇（DEG）与水的比例，并使用了三种不同的铈前驱体盐（Ce(NO)、CeCl和Ce(CHCOO)）。对合成的CeO NPs的结构、尺寸和形态进行了研究。通过XRD分析得到平均微晶尺寸为13至33nm。合成的CeO NPs呈现出球形和细长形形态。通过改变DEG和水的不同比例，得到平均粒径在16 - 36nm范围内。使用FTIR证实了CeO NPs表面存在DEG分子。合成的CeO NPs用于研究抗糖尿病和细胞活力（细胞毒性）特性。通过α - 葡萄糖苷酶抑制活性进行抗糖尿病研究。使用Ce(NO)和CeCl前驱体合成的CeO显示出约40.0%的α - 葡萄糖苷酶抑制活性，而使用Ce(CHCOO)合成的CeO显示出最低的α - 葡萄糖苷酶抑制活性。使用细胞毒性试验研究了CeO NPs的细胞活力特性。使用Ce(NO)和CeCl制备的CeO NPs在较低浓度下无毒，而使用Ce(CHCOO)制备的CeO NPs在所有浓度下均无毒。因此，多元醇介导合成的CeO NPs表现出相当好的α - 葡萄糖苷酶抑制活性和生物相容性。