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梅(Prunus mume Sieb. et Zucc.)果实的酚类物质概况

Phenolics profile of mume, Japanese apricot (Prunus mume Sieb. et Zucc.) fruit.

作者信息

Mitani Takahiko, Horinishi Asako, Kishida Kunihiro, Kawabata Tomoaki, Yano Fumiko, Mimura Hisa, Inaba Nobuya, Yamanishi Hisako, Oe Takaaki, Negoro Keiichi, Mori Hajime, Miyake Yasuhito, Hosoda Asao, Tanaka Yoshie, Mori Megumi, Ozaki Yoshihiko

机构信息

Faculty of Biology-oriented Science and Technology, Kinki University.

出版信息

Biosci Biotechnol Biochem. 2013;77(8):1623-7. doi: 10.1271/bbb.130077. Epub 2013 Aug 7.

Abstract

The fruit of mume, Japanese apricot (Prunus mume Sieb. et Zucc.), was evaluated for its phenolics content, high performance liquid chromatography (HPLC) profile and antioxidative activities. The phenolics content of mume fruit was relatively high, the flesh of fully matured fruit containing up to 1% of phenolics on a dry weight basis. Reflecting such a high content of phenolics, the ORAC (oxygen radical absorbance capacity) value for mume fruit flesh showed high values, ranging from 150 to 320 µmol/g Trolox equivalent, depending upon the stage of maturation. 5-O-Caffeoylqunic acid (chlorogenic acid), 3-O-caffeoylquinic acid and tetra-O-acylated sucrose-related compounds were isolated from the flesh of mume fruit, although many unknown peaks were also apparent in the HPLC chromatogram. An alkali hydrolysate comprised four main phenolic acids, caffeic acid, cis/trans-p-coumaric acid and ferulic acid. No flavonoids were observed in the analysis. These results suggest that the majority of phenolics in mume fruit were hydroxycinnamic acid derivatives.

摘要

对青梅(Prunus mume Sieb. et Zucc.)果实的酚类物质含量、高效液相色谱(HPLC)图谱及抗氧化活性进行了评估。青梅果实的酚类物质含量相对较高,完全成熟果实的果肉干重中酚类物质含量高达1%。鉴于酚类物质含量如此之高,青梅果肉的氧自由基吸收能力(ORAC)值较高,根据成熟阶段不同,范围在150至320 μmol/g Trolox当量之间。从青梅果肉中分离出了5-O-咖啡酰奎尼酸(绿原酸)、3-O-咖啡酰奎尼酸和四-O-酰化蔗糖相关化合物,不过在HPLC色谱图中也出现了许多未知峰。一种碱水解产物包含四种主要酚酸,即咖啡酸、顺式/反式对香豆酸和阿魏酸。分析中未观察到黄酮类化合物。这些结果表明,青梅果实中的大多数酚类物质为羟基肉桂酸衍生物。

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